miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
11198 3' -45.8 NC_002816.1 + 118070 0.68 0.99939
Target:  5'- aGUuuGGCUGUggACGAACAUgcgcgcgacgaagCGUACGa -3'
miRNA:   3'- gCAggUUGACA--UGCUUGUAa------------GCAUGU- -5'
11198 3' -45.8 NC_002816.1 + 84098 0.66 0.999946
Target:  5'- uGUCCAuUUGUcgucaACGGugGaUCGUACAc -3'
miRNA:   3'- gCAGGUuGACA-----UGCUugUaAGCAUGU- -5'
11198 3' -45.8 NC_002816.1 + 77775 0.71 0.994604
Target:  5'- uCGUgaaCGG-UGUACGAAUGUUCGUACAg -3'
miRNA:   3'- -GCAg--GUUgACAUGCUUGUAAGCAUGU- -5'
11198 3' -45.8 NC_002816.1 + 77617 1.1 0.023024
Target:  5'- uCGUCCAACUGUACGAACAUUCGUACAc -3'
miRNA:   3'- -GCAGGUUGACAUGCUUGUAAGCAUGU- -5'
11198 3' -45.8 NC_002816.1 + 60713 0.67 0.999873
Target:  5'- --aCCAugUGUACGAACcgUaucgUGUGCu -3'
miRNA:   3'- gcaGGUugACAUGCUUGuaA----GCAUGu -5'
11198 3' -45.8 NC_002816.1 + 17440 0.67 0.999904
Target:  5'- --cCCAACUGgccACGGACGUUgGUGa- -3'
miRNA:   3'- gcaGGUUGACa--UGCUUGUAAgCAUgu -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.