Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 112 | 3' | -55.9 | AC_000006.1 | + | 8817 | 0.66 | 0.559392 |
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Target: 5'- gCAGGUggucaugcgcGCGCGcGAcgccgacggggacguGGUCUACAGCCgCGu -3' miRNA: 3'- -GUCCA----------UGCGC-CU---------------UCAGGUGUUGGgGC- -5' |
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| 112 | 3' | -55.9 | AC_000006.1 | + | 30661 | 0.66 | 0.533042 |
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Target: 5'- gAGGU-CGCGG-AGUCCGCAAagggUCuuGa -3' miRNA: 3'- gUCCAuGCGCCuUCAGGUGUU----GGggC- -5' |
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| 112 | 3' | -55.9 | AC_000006.1 | + | 23878 | 0.67 | 0.511429 |
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Target: 5'- gAGGcGCgGCGGGAcuauGUCCGCGACUgCGu -3' miRNA: 3'- gUCCaUG-CGCCUU----CAGGUGUUGGgGC- -5' |
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| 112 | 3' | -55.9 | AC_000006.1 | + | 17315 | 0.69 | 0.40981 |
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Target: 5'- -uGGUuCGCGau-GUCCACcACCCCGu -3' miRNA: 3'- guCCAuGCGCcuuCAGGUGuUGGGGC- -5' |
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| 112 | 3' | -55.9 | AC_000006.1 | + | 15589 | 0.69 | 0.391026 |
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Target: 5'- uGGGUGCGCgacuccgucacGGgcGUgCGCGugCCCGu -3' miRNA: 3'- gUCCAUGCG-----------CCuuCAgGUGUugGGGC- -5' |
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| 112 | 3' | -55.9 | AC_000006.1 | + | 1968 | 0.69 | 0.381848 |
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Target: 5'- aCAGGUGCaugGCuGcGAAGUCCAgaaugUAGCCCCu -3' miRNA: 3'- -GUCCAUG---CG-C-CUUCAGGU-----GUUGGGGc -5' |
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| 112 | 3' | -55.9 | AC_000006.1 | + | 24439 | 0.74 | 0.174631 |
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Target: 5'- aAGGUcCGCaGGAGUCCACcGCUCCGc -3' miRNA: 3'- gUCCAuGCGcCUUCAGGUGuUGGGGC- -5' |
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| 112 | 3' | -55.9 | AC_000006.1 | + | 24538 | 1.09 | 0.000504 |
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Target: 5'- gCAGGUACGCGGAAGUCCACAACCCCGg -3' miRNA: 3'- -GUCCAUGCGCCUUCAGGUGUUGGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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