Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1120 | 5' | -51.4 | NC_000942.1 | + | 14047 | 0.67 | 0.708002 |
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Target: 5'- aAGCGCUcuGCCGaGCAU----CCCGUUCa -3' miRNA: 3'- -UCGUGG--CGGCaUGUAaaauGGGCAGG- -5' |
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| 1120 | 5' | -51.4 | NC_000942.1 | + | 24725 | 0.67 | 0.701113 |
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Target: 5'- aAGCGCaGCCGaucuggACGgugaaucagucaUACCCGUCCa -3' miRNA: 3'- -UCGUGgCGGCa-----UGUaaa---------AUGGGCAGG- -5' |
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| 1120 | 5' | -51.4 | NC_000942.1 | + | 15078 | 0.68 | 0.661638 |
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Target: 5'- aGGCgucGCCGCCGUcgACGUagaucccCUCGUCCu -3' miRNA: 3'- -UCG---UGGCGGCA--UGUAaaau---GGGCAGG- -5' |
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| 1120 | 5' | -51.4 | NC_000942.1 | + | 15178 | 0.69 | 0.62649 |
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Target: 5'- cAGUACCgGuuGUACGUUaucACCCcUCCa -3' miRNA: 3'- -UCGUGG-CggCAUGUAAaa-UGGGcAGG- -5' |
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| 1120 | 5' | -51.4 | NC_000942.1 | + | 23345 | 0.7 | 0.522499 |
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Target: 5'- uGGaCGCUGUCGcugucUGCGcugUACCCGUCCg -3' miRNA: 3'- -UC-GUGGCGGC-----AUGUaaaAUGGGCAGG- -5' |
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| 1120 | 5' | -51.4 | NC_000942.1 | + | 14074 | 1.12 | 0.000805 |
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Target: 5'- gAGCACCGCCGUACAUUUUACCCGUCCc -3' miRNA: 3'- -UCGUGGCGGCAUGUAAAAUGGGCAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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