Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11202 | 5' | -49.7 | NC_002816.1 | + | 78926 | 0.72 | 0.896533 |
Target: 5'- uCugGGgcUAGUUUCGCCGUCCAuguUGUGCa -3' miRNA: 3'- uGugUU--AUUAGAGUGGCAGGU---GCGCG- -5' |
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11202 | 5' | -49.7 | NC_002816.1 | + | 116180 | 0.72 | 0.896533 |
Target: 5'- aACACAAUuuuuAUCUgGCCGUUggUAUGUGCg -3' miRNA: 3'- -UGUGUUAu---UAGAgUGGCAG--GUGCGCG- -5' |
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11202 | 5' | -49.7 | NC_002816.1 | + | 75113 | 0.73 | 0.866966 |
Target: 5'- aACAUAGUGGUCUU--UGUCCACGCa- -3' miRNA: 3'- -UGUGUUAUUAGAGugGCAGGUGCGcg -5' |
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11202 | 5' | -49.7 | NC_002816.1 | + | 13581 | 0.74 | 0.806488 |
Target: 5'- gACACGGUAGuguUCUCACCGgguaaCACGCa- -3' miRNA: 3'- -UGUGUUAUU---AGAGUGGCag---GUGCGcg -5' |
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11202 | 5' | -49.7 | NC_002816.1 | + | 93068 | 1.12 | 0.006009 |
Target: 5'- aACACAAUAAUCUCACCGUCCACGCGCu -3' miRNA: 3'- -UGUGUUAUUAGAGUGGCAGGUGCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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