Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11217 | 3' | -39.9 | NC_003038.1 | + | 134361 | 0.69 | 1 |
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Target: 5'- ----aGUUUGucc-CAAAAAGGUGGGg -3' miRNA: 3'- ggaaaUAGACuuuaGUUUUUCCACCC- -5' |
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| 11217 | 3' | -39.9 | NC_003038.1 | + | 179763 | 0.69 | 1 |
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Target: 5'- aUUUUucCUGAuauUCAAAGAGuGUGGGa -3' miRNA: 3'- gGAAAuaGACUuu-AGUUUUUC-CACCC- -5' |
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| 11217 | 3' | -39.9 | NC_003038.1 | + | 63750 | 0.68 | 1 |
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Target: 5'- uCCUggAUUUGGAuuagcUgGAGGAGGUGGa -3' miRNA: 3'- -GGAaaUAGACUUu----AgUUUUUCCACCc -5' |
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| 11217 | 3' | -39.9 | NC_003038.1 | + | 37865 | 0.68 | 1 |
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Target: 5'- aCUUUAUCcGAAguuaaucaguuAUCGAuguuagaggaggaguAGGGGUGGGu -3' miRNA: 3'- gGAAAUAGaCUU-----------UAGUU---------------UUUCCACCC- -5' |
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| 11217 | 3' | -39.9 | NC_003038.1 | + | 205348 | 0.66 | 1 |
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Target: 5'- --aUUAUUUGAGAUaAAGGAGGUGa- -3' miRNA: 3'- ggaAAUAGACUUUAgUUUUUCCACcc -5' |
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| 11217 | 3' | -39.9 | NC_003038.1 | + | 134633 | 1.19 | 0.058002 |
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Target: 5'- aCCUUUAUCUGAAAUCAAAAAGGUGGGg -3' miRNA: 3'- -GGAAAUAGACUUUAGUUUUUCCACCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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