miRNA: 3'- gCUGGACUUUuGaGAC--UGUGGUCugG- -5'
miRNA: 3'- -GCUGGACUUuugAGacuGUGGUCUGG- -5'
miRNA: 3'- gCUGGACUUuuGa---GACuGUGGUc--UGG- -5'
miRNA: 3'- gCUGGACUUUUGAG---------------ACUGUGGuCUGG- -5'
miRNA: 3'- -GCUGGACUUUUGAGACUGUGGUCUGG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11218 | 5' | -49.4 | NC_003038.1 | + | 203864 | 0.66 | 0.99966 |
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Target: 5'- aGAUUUGAAAuC-CUGaaGCACCAGugCu -3' miRNA: 3'- gCUGGACUUUuGaGAC--UGUGGUCugG- -5' |
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| 11218 | 5' | -49.4 | NC_003038.1 | + | 183100 | 0.67 | 0.998817 |
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Target: 5'- aCGAUCUGAA---UCgu-CAUCAGACCa -3' miRNA: 3'- -GCUGGACUUuugAGacuGUGGUCUGG- -5' |
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| 11218 | 5' | -49.4 | NC_003038.1 | + | 63838 | 0.69 | 0.99537 |
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Target: 5'- gGACCUGGAggUggugCUG-CACCAacuGCCg -3' miRNA: 3'- gCUGGACUUuuGa---GACuGUGGUc--UGG- -5' |
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| 11218 | 5' | -49.4 | NC_003038.1 | + | 146402 | 0.7 | 0.989829 |
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Target: 5'- uGGCCUGAAAAUUUagcuuuauacaaagaUGAUGCCuGACUu -3' miRNA: 3'- gCUGGACUUUUGAG---------------ACUGUGGuCUGG- -5' |
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| 11218 | 5' | -49.4 | NC_003038.1 | + | 157989 | 1.13 | 0.010641 |
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Target: 5'- gCGACCUGAAAACUCUGACACCAGACCu -3' miRNA: 3'- -GCUGGACUUUUGAGACUGUGGUCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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