Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11225 | 5' | -40.6 | NC_003038.1 | + | 202204 | 0.69 | 1 |
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Target: 5'- --aGCAGgUGGUACAAUGuCUGgaaAUAUUa -3' miRNA: 3'- gaaCGUCaACCAUGUUAU-GAU---UAUAA- -5' |
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| 11225 | 5' | -40.6 | NC_003038.1 | + | 122490 | 0.66 | 1 |
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Target: 5'- -gUGCAGUUGGagcUACAcaACUAGg--- -3' miRNA: 3'- gaACGUCAACC---AUGUuaUGAUUauaa -5' |
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| 11225 | 5' | -40.6 | NC_003038.1 | + | 167544 | 0.66 | 1 |
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Target: 5'- aUUGCGGgaaUGGUGCAcuucCUAAUAa- -3' miRNA: 3'- gAACGUCa--ACCAUGUuau-GAUUAUaa -5' |
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| 11225 | 5' | -40.6 | NC_003038.1 | + | 123275 | 0.69 | 1 |
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Target: 5'- aCUUGCAG-UGGuUACAggGCUAGUu-- -3' miRNA: 3'- -GAACGUCaACC-AUGUuaUGAUUAuaa -5' |
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| 11225 | 5' | -40.6 | NC_003038.1 | + | 64078 | 0.68 | 1 |
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Target: 5'- --gGCAGUUGGUGCAGcACc------ -3' miRNA: 3'- gaaCGUCAACCAUGUUaUGauuauaa -5' |
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| 11225 | 5' | -40.6 | NC_003038.1 | + | 128977 | 0.67 | 1 |
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Target: 5'- -cUGCuaaAGUUGGUGC--UGCUGGUAc- -3' miRNA: 3'- gaACG---UCAACCAUGuuAUGAUUAUaa -5' |
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| 11225 | 5' | -40.6 | NC_003038.1 | + | 129806 | 0.66 | 1 |
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Target: 5'- --gGCAGUUGGUuACGAUAauaUGAUu-- -3' miRNA: 3'- gaaCGUCAACCA-UGUUAUg--AUUAuaa -5' |
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| 11225 | 5' | -40.6 | NC_003038.1 | + | 196983 | 0.83 | 0.970623 |
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Target: 5'- uCUUGCAGgUGGUACAAUgACUGGUAc- -3' miRNA: 3'- -GAACGUCaACCAUGUUA-UGAUUAUaa -5' |
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| 11225 | 5' | -40.6 | NC_003038.1 | + | 128770 | 1.08 | 0.129248 |
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Target: 5'- gCUUGCAGUUGGUACAAUACUAAUAUUg -3' miRNA: 3'- -GAACGUCAACCAUGUUAUGAUUAUAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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