Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11226 | 5' | -45.8 | NC_003038.1 | + | 165067 | 0.66 | 0.999996 |
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Target: 5'- cCCCUCCACCUg-UUGGUga-AGAa-- -3' miRNA: 3'- -GGGGGGUGGAaaAACUAaagUCUauu -5' |
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| 11226 | 5' | -45.8 | NC_003038.1 | + | 185224 | 0.67 | 0.999984 |
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Target: 5'- aCCCCCCugCUUUUUGc-----GGUAAc -3' miRNA: 3'- -GGGGGGugGAAAAACuaaaguCUAUU- -5' |
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| 11226 | 5' | -45.8 | NC_003038.1 | + | 109521 | 0.67 | 0.999978 |
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Target: 5'- gCCCCCCACCg----GAaaUUUCcaacagaagAGAUGAa -3' miRNA: 3'- -GGGGGGUGGaaaaaCU--AAAG---------UCUAUU- -5' |
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| 11226 | 5' | -45.8 | NC_003038.1 | + | 185019 | 0.71 | 0.998488 |
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Target: 5'- aCCCCCCugCUUUUUaauugguuggGAUUUCAa---- -3' miRNA: 3'- -GGGGGGugGAAAAA----------CUAAAGUcuauu -5' |
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| 11226 | 5' | -45.8 | NC_003038.1 | + | 121741 | 0.74 | 0.988011 |
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Target: 5'- aUCCCCCACCUccacUUUUGAg---AGAUGAa -3' miRNA: 3'- -GGGGGGUGGA----AAAACUaaagUCUAUU- -5' |
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| 11226 | 5' | -45.8 | NC_003038.1 | + | 134597 | 0.76 | 0.966333 |
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Target: 5'- gCCCCCCACCUUUUUGGga-CAa---- -3' miRNA: 3'- -GGGGGGUGGAAAAACUaaaGUcuauu -5' |
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| 11226 | 5' | -45.8 | NC_003038.1 | + | 134391 | 1.1 | 0.033285 |
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Target: 5'- gCCCCCCACCUUUUUGAUUUCAGAUAAa -3' miRNA: 3'- -GGGGGGUGGAAAAACUAAAGUCUAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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