Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11227 | 3' | -47.8 | NC_003038.1 | + | 125227 | 0.66 | 0.999968 |
Target: 5'- cAGGUCua-UCUGAGGgagcgguuguguugGGAUUAUUUCCa -3' miRNA: 3'- -UCUAGuagAGGCUCU--------------UCUAGUAGAGG- -5' |
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11227 | 3' | -47.8 | NC_003038.1 | + | 12265 | 0.66 | 0.999948 |
Target: 5'- gAGGUCAUUUUgGAGGAGGUUuauAUagUCCa -3' miRNA: 3'- -UCUAGUAGAGgCUCUUCUAG---UAg-AGG- -5' |
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11227 | 3' | -47.8 | NC_003038.1 | + | 41276 | 0.66 | 0.999931 |
Target: 5'- uGAUaCAUUUCCaacGAAGAuuUCAUUUCCa -3' miRNA: 3'- uCUA-GUAGAGGcu-CUUCU--AGUAGAGG- -5' |
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11227 | 3' | -47.8 | NC_003038.1 | + | 39522 | 0.66 | 0.999931 |
Target: 5'- -cGUCGUCUCgGGGcucaGUCGUUUCCa -3' miRNA: 3'- ucUAGUAGAGgCUCuuc-UAGUAGAGG- -5' |
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11227 | 3' | -47.8 | NC_003038.1 | + | 43038 | 0.67 | 0.999753 |
Target: 5'- ---aCGUCUCCuuGggGAUCuucuaguagAUCUCCu -3' miRNA: 3'- ucuaGUAGAGGcuCuuCUAG---------UAGAGG- -5' |
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11227 | 3' | -47.8 | NC_003038.1 | + | 167497 | 0.68 | 0.999252 |
Target: 5'- gAGAUgAUCUUCucGGAGAUgAUCUUCu -3' miRNA: 3'- -UCUAgUAGAGGcuCUUCUAgUAGAGG- -5' |
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11227 | 3' | -47.8 | NC_003038.1 | + | 108053 | 0.72 | 0.984619 |
Target: 5'- aAGAagccCAUCUCCuAGAAGAagucCAUCUCCu -3' miRNA: 3'- -UCUa---GUAGAGGcUCUUCUa---GUAGAGG- -5' |
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11227 | 3' | -47.8 | NC_003038.1 | + | 96281 | 0.79 | 0.820279 |
Target: 5'- aAGGUCGUCUCCcccacaugGGGGAGAcgAUCUCCa -3' miRNA: 3'- -UCUAGUAGAGG--------CUCUUCUagUAGAGG- -5' |
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11227 | 3' | -47.8 | NC_003038.1 | + | 184787 | 0.79 | 0.802239 |
Target: 5'- ----gAUCUCCGGGAAGAUCGUCUa- -3' miRNA: 3'- ucuagUAGAGGCUCUUCUAGUAGAgg -5' |
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11227 | 3' | -47.8 | NC_003038.1 | + | 167332 | 0.99 | 0.106522 |
Target: 5'- aAGGUCAUCUCCuAGAAGGUCAUCUCCu -3' miRNA: 3'- -UCUAGUAGAGGcUCUUCUAGUAGAGG- -5' |
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11227 | 3' | -47.8 | NC_003038.1 | + | 167302 | 1 | 0.090475 |
Target: 5'- aAGAUCAUCUCCuAGAAGGUCAUCUCCu -3' miRNA: 3'- -UCUAGUAGAGGcUCUUCUAGUAGAGG- -5' |
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11227 | 3' | -47.8 | NC_003038.1 | + | 167260 | 1.15 | 0.012664 |
Target: 5'- aAGAUCAUCUCCGAGAAGAUCAUCUCCg -3' miRNA: 3'- -UCUAGUAGAGGCUCUUCUAGUAGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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