Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11227 | 5' | -48.3 | NC_003038.1 | + | 12262 | 0.66 | 0.99961 |
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Target: 5'- uAGGAGGUCAUUUUggAGGAGGUUu- -3' miRNA: 3'- uUCUUCUAGUAGAGgaUCUUCCAGua -5' |
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| 11227 | 5' | -48.3 | NC_003038.1 | + | 167484 | 0.68 | 0.995959 |
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Target: 5'- -cGGAGAUgAUCUUCUuggAGGAGGUCc- -3' miRNA: 3'- uuCUUCUAgUAGAGGA---UCUUCCAGua -5' |
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| 11227 | 5' | -48.3 | NC_003038.1 | + | 108052 | 0.71 | 0.979138 |
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Target: 5'- gAAGAAGccCAUCUCCUAGAAGaaGUCc- -3' miRNA: 3'- -UUCUUCuaGUAGAGGAUCUUC--CAGua -5' |
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| 11227 | 5' | -48.3 | NC_003038.1 | + | 167271 | 0.9 | 0.215405 |
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Target: 5'- cGAGAAGAUCAUCUCCgAGAAGGUCu- -3' miRNA: 3'- -UUCUUCUAGUAGAGGaUCUUCCAGua -5' |
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| 11227 | 5' | -48.3 | NC_003038.1 | + | 167329 | 1 | 0.05893 |
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Target: 5'- uAGAAGGUCAUCUCCUAGAAGGUCAUc -3' miRNA: 3'- uUCUUCUAGUAGAGGAUCUUCCAGUA- -5' |
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| 11227 | 5' | -48.3 | NC_003038.1 | + | 167298 | 1.03 | 0.038288 |
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Target: 5'- cAAGAAGAUCAUCUCCUAGAAGGUCAUc -3' miRNA: 3'- -UUCUUCUAGUAGAGGAUCUUCCAGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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