Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1124 | 5' | -54.7 | NC_000942.1 | + | 4394 | 0.66 | 0.616013 |
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Target: 5'- uGUUCCGGGGAUuggggugGGCAGUgGa-- -3' miRNA: 3'- gCAGGGCCCUUGuaa----CCGUCAgCagg -5' |
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| 1124 | 5' | -54.7 | NC_000942.1 | + | 30047 | 0.74 | 0.217616 |
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Target: 5'- uGUgCCCGGGGACAUgggacuUGGCGGUgUGcCCg -3' miRNA: 3'- gCA-GGGCCCUUGUA------ACCGUCA-GCaGG- -5' |
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| 1124 | 5' | -54.7 | NC_000942.1 | + | 29966 | 0.75 | 0.194949 |
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Target: 5'- uGUgCCCGGGGACAUgggacUGGCGGUgUGcCCg -3' miRNA: 3'- gCA-GGGCCCUUGUA-----ACCGUCA-GCaGG- -5' |
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| 1124 | 5' | -54.7 | NC_000942.1 | + | 29870 | 0.75 | 0.189614 |
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Target: 5'- uGUgCCCGGGAACAUUGGCAGn----- -3' miRNA: 3'- gCA-GGGCCCUUGUAACCGUCagcagg -5' |
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| 1124 | 5' | -54.7 | NC_000942.1 | + | 29913 | 0.93 | 0.008923 |
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Target: 5'- uGUgCCCGGGAACAUUGGCAGUCGgCCc -3' miRNA: 3'- gCA-GGGCCCUUGUAACCGUCAGCaGG- -5' |
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| 1124 | 5' | -54.7 | NC_000942.1 | + | 29849 | 1.11 | 0.00046 |
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Target: 5'- uCGUCCCGGGAACAUUGGCAGUCGUCCc -3' miRNA: 3'- -GCAGGGCCCUUGUAACCGUCAGCAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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