Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1127 | 5' | -58 | NC_000942.1 | + | 23468 | 0.66 | 0.349114 |
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Target: 5'- gUGAUGGuGGUGGUggugGCAGUCGCauucgagGGCg -3' miRNA: 3'- aAUUACC-UCGCCGa---CGUCGGCG-------UCGa -5' |
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| 1127 | 5' | -58 | NC_000942.1 | + | 14835 | 0.66 | 0.332609 |
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Target: 5'- --cGUGGcAGCuGUgGCAGCCGCAGg- -3' miRNA: 3'- aauUACC-UCGcCGaCGUCGGCGUCga -5' |
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| 1127 | 5' | -58 | NC_000942.1 | + | 23400 | 0.69 | 0.210388 |
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Target: 5'- ---cUGGAGC-GCUGCAGCCGUcuGGg- -3' miRNA: 3'- aauuACCUCGcCGACGUCGGCG--UCga -5' |
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| 1127 | 5' | -58 | NC_000942.1 | + | 16246 | 0.7 | 0.167576 |
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Target: 5'- gUGcgGGAacgGCuauGGCUGCGGgCGCGGCUa -3' miRNA: 3'- aAUuaCCU---CG---CCGACGUCgGCGUCGA- -5' |
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| 1127 | 5' | -58 | NC_000942.1 | + | 9514 | 0.71 | 0.144944 |
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Target: 5'- -cAGUGGcgcGUGGCUGgGGcCCGCGGCg -3' miRNA: 3'- aaUUACCu--CGCCGACgUC-GGCGUCGa -5' |
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| 1127 | 5' | -58 | NC_000942.1 | + | 14359 | 0.75 | 0.070683 |
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Target: 5'- ---uUGGAgcuGCGGCUGCAGCCGCuccauuaacuGCUc -3' miRNA: 3'- aauuACCU---CGCCGACGUCGGCGu---------CGA- -5' |
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| 1127 | 5' | -58 | NC_000942.1 | + | 14299 | 1.05 | 0.000339 |
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Target: 5'- gUUAAUGGAGCGGCUGCAGCCGCAGCUc -3' miRNA: 3'- -AAUUACCUCGCCGACGUCGGCGUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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