Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11280 | 5' | -42.4 | NC_003083.1 | + | 28884 | 0.66 | 1 |
Target: 5'- uGGAUGUugcaacgcgaAAAGCGCGGUaauccacacgGUGGCGUGc -3' miRNA: 3'- uUUUACA----------UUUUGUGCUA----------CACCGUACu -5' |
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11280 | 5' | -42.4 | NC_003083.1 | + | 66908 | 0.66 | 1 |
Target: 5'- aAGGAUGUAAAA-ACGAuuucaaauguugUGUGGCGUa- -3' miRNA: 3'- -UUUUACAUUUUgUGCU------------ACACCGUAcu -5' |
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11280 | 5' | -42.4 | NC_003083.1 | + | 106692 | 0.67 | 0.999996 |
Target: 5'- cAGGUGUGAguaguccuGACACcAUGUGGCGcaauUGAa -3' miRNA: 3'- uUUUACAUU--------UUGUGcUACACCGU----ACU- -5' |
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11280 | 5' | -42.4 | NC_003083.1 | + | 87700 | 0.68 | 0.999995 |
Target: 5'- cGAAGUGgcGGACGCGGacGUGGuCGUGc -3' miRNA: 3'- -UUUUACauUUUGUGCUa-CACC-GUACu -5' |
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11280 | 5' | -42.4 | NC_003083.1 | + | 73622 | 0.68 | 0.999989 |
Target: 5'- uAAAAUGUuagAAAGCGCGuuugacgaaGUGGCAUGc -3' miRNA: 3'- -UUUUACA---UUUUGUGCua-------CACCGUACu -5' |
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11280 | 5' | -42.4 | NC_003083.1 | + | 9089 | 0.68 | 0.999984 |
Target: 5'- cAAAUGUGccAAGCGCcgcacguuugGGUGUGGCAaaUGAu -3' miRNA: 3'- uUUUACAU--UUUGUG----------CUACACCGU--ACU- -5' |
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11280 | 5' | -42.4 | NC_003083.1 | + | 13360 | 0.7 | 0.999813 |
Target: 5'- gAAAcgGgcGAAUGCGAuUGUGGCgAUGAa -3' miRNA: 3'- -UUUuaCauUUUGUGCU-ACACCG-UACU- -5' |
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11280 | 5' | -42.4 | NC_003083.1 | + | 34729 | 0.74 | 0.995164 |
Target: 5'- ----gGUGAgcAGCGCGuugauUGUGGCGUGAa -3' miRNA: 3'- uuuuaCAUU--UUGUGCu----ACACCGUACU- -5' |
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11280 | 5' | -42.4 | NC_003083.1 | + | 19020 | 0.86 | 0.640749 |
Target: 5'- gAAAAUGUAAAACACG-UGUGGgAUGAu -3' miRNA: 3'- -UUUUACAUUUUGUGCuACACCgUACU- -5' |
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11280 | 5' | -42.4 | NC_003083.1 | + | 19133 | 1.08 | 0.05052 |
Target: 5'- aAAAAUGUAAAACACGAUGUGGCAUGAc -3' miRNA: 3'- -UUUUACAUUUUGUGCUACACCGUACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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