Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11281 | 3' | -56.6 | NC_003083.1 | + | 108155 | 0.66 | 0.807983 |
Target: 5'- gGGUUGgC-GCGCAUCGUCGcguugaGCGGAUu -3' miRNA: 3'- gCUAACgGuUGCGUGGCAGC------CGCCUA- -5' |
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11281 | 3' | -56.6 | NC_003083.1 | + | 51481 | 0.67 | 0.798963 |
Target: 5'- -aAUUGCCAACGCACCGUacaaacGCGu-- -3' miRNA: 3'- gcUAACGGUUGCGUGGCAgc----CGCcua -5' |
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11281 | 3' | -56.6 | NC_003083.1 | + | 107692 | 0.67 | 0.761426 |
Target: 5'- ----cGCCAAuCGCACCGgCGGCGa-- -3' miRNA: 3'- gcuaaCGGUU-GCGUGGCaGCCGCcua -5' |
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11281 | 3' | -56.6 | NC_003083.1 | + | 43721 | 0.67 | 0.761426 |
Target: 5'- -cGUUGgCAACGCAuugaucCCGUCGGCGa-- -3' miRNA: 3'- gcUAACgGUUGCGU------GGCAGCCGCcua -5' |
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11281 | 3' | -56.6 | NC_003083.1 | + | 23115 | 0.68 | 0.732004 |
Target: 5'- ---aUGCCGgcgGCGCGCUGUUGGCGu-- -3' miRNA: 3'- gcuaACGGU---UGCGUGGCAGCCGCcua -5' |
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11281 | 3' | -56.6 | NC_003083.1 | + | 112331 | 0.69 | 0.639954 |
Target: 5'- gGGUuuUGCUguAACGCgcgaucgacACCGUCGGUGGAg -3' miRNA: 3'- gCUA--ACGG--UUGCG---------UGGCAGCCGCCUa -5' |
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11281 | 3' | -56.6 | NC_003083.1 | + | 24313 | 0.7 | 0.598488 |
Target: 5'- uGAUUGgCGACGCAgcucUCGUCGGCGu-- -3' miRNA: 3'- gCUAACgGUUGCGU----GGCAGCCGCcua -5' |
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11281 | 3' | -56.6 | NC_003083.1 | + | 84588 | 0.71 | 0.577877 |
Target: 5'- gCGAcaGCgcaGACGCACgCGUCGGCGGu- -3' miRNA: 3'- -GCUaaCGg--UUGCGUG-GCAGCCGCCua -5' |
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11281 | 3' | -56.6 | NC_003083.1 | + | 74268 | 0.71 | 0.537184 |
Target: 5'- uGAacGCCGACGaCGCCG-CGGCGGu- -3' miRNA: 3'- gCUaaCGGUUGC-GUGGCaGCCGCCua -5' |
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11281 | 3' | -56.6 | NC_003083.1 | + | 115269 | 0.75 | 0.331461 |
Target: 5'- aCGAgcUGCCaGugGCGCCGUUGGCGGc- -3' miRNA: 3'- -GCUa-ACGG-UugCGUGGCAGCCGCCua -5' |
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11281 | 3' | -56.6 | NC_003083.1 | + | 24190 | 1.06 | 0.002806 |
Target: 5'- cCGAUUGCCAACGCACCGUCGGCGGAUg -3' miRNA: 3'- -GCUAACGGUUGCGUGGCAGCCGCCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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