Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11281 | 5' | -50.7 | NC_003083.1 | + | 72824 | 0.66 | 0.987123 |
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Target: 5'- uCGucgGCGUCGACGG-GUGUa-CGUGCc -3' miRNA: 3'- -GCua-UGCAGCUGCCgCACAcaGUAUG- -5' |
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| 11281 | 5' | -50.7 | NC_003083.1 | + | 71614 | 0.67 | 0.985418 |
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Target: 5'- gGAUGCGUUu-UGGUGuUGUgGUCGUACg -3' miRNA: 3'- gCUAUGCAGcuGCCGC-ACA-CAGUAUG- -5' |
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| 11281 | 5' | -50.7 | NC_003083.1 | + | 74274 | 0.67 | 0.983546 |
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Target: 5'- cCGAcgACGcCG-CGGCGgUGUGUCAg-- -3' miRNA: 3'- -GCUa-UGCaGCuGCCGC-ACACAGUaug -5' |
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| 11281 | 5' | -50.7 | NC_003083.1 | + | 12076 | 0.67 | 0.981497 |
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Target: 5'- gCGAU-CGgUGACaauGGCGUucGUGUCGUGCg -3' miRNA: 3'- -GCUAuGCaGCUG---CCGCA--CACAGUAUG- -5' |
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| 11281 | 5' | -50.7 | NC_003083.1 | + | 116940 | 0.69 | 0.945 |
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Target: 5'- uGGUACGcUGugGGUGUGUuGaCGUACa -3' miRNA: 3'- gCUAUGCaGCugCCGCACA-CaGUAUG- -5' |
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| 11281 | 5' | -50.7 | NC_003083.1 | + | 24153 | 1.11 | 0.006563 |
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Target: 5'- cCGAUACGUCGACGGCGUGUGUCAUACu -3' miRNA: 3'- -GCUAUGCAGCUGCCGCACACAGUAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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