Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11282 | 5' | -57.9 | NC_003083.1 | + | 50174 | 0.66 | 0.790559 |
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Target: 5'- uCGGCCGuuucuagucGCGUCGUUUGcCCAGCa--- -3' miRNA: 3'- -GCCGGU---------CGCGGCAAACaGGUUGccga -5' |
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| 11282 | 5' | -57.9 | NC_003083.1 | + | 74191 | 0.66 | 0.787805 |
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Target: 5'- gCGGCCGuuugcGCGuuGUUuauauugugcaacaUGUCCG-CGGCc -3' miRNA: 3'- -GCCGGU-----CGCggCAA--------------ACAGGUuGCCGa -5' |
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| 11282 | 5' | -57.9 | NC_003083.1 | + | 24075 | 0.66 | 0.751898 |
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Target: 5'- uGGCCAGCacacaaaucuuucGCCGUUUGgCCAAaaGUa -3' miRNA: 3'- gCCGGUCG-------------CGGCAAACaGGUUgcCGa -5' |
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| 11282 | 5' | -57.9 | NC_003083.1 | + | 51286 | 0.67 | 0.693287 |
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Target: 5'- aCGaGCgC-GCGCgCGUUUGUuuGACGGCg -3' miRNA: 3'- -GC-CG-GuCGCG-GCAAACAggUUGCCGa -5' |
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| 11282 | 5' | -57.9 | NC_003083.1 | + | 72786 | 0.71 | 0.472015 |
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Target: 5'- -cGCCGGCGCgCGUUUGacuuucaaCAACGGCa -3' miRNA: 3'- gcCGGUCGCG-GCAAACag------GUUGCCGa -5' |
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| 11282 | 5' | -57.9 | NC_003083.1 | + | 70029 | 0.71 | 0.471072 |
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Target: 5'- uGGCCAGCGUCGcaggccgUGUCUuucacugGAUGGCa -3' miRNA: 3'- gCCGGUCGCGGCaa-----ACAGG-------UUGCCGa -5' |
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| 11282 | 5' | -57.9 | NC_003083.1 | + | 27714 | 0.75 | 0.291892 |
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Target: 5'- uGGCCGGCGCCGcaaUGUCCggUuGGUg -3' miRNA: 3'- gCCGGUCGCGGCaa-ACAGGuuG-CCGa -5' |
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| 11282 | 5' | -57.9 | NC_003083.1 | + | 32296 | 1.08 | 0.001696 |
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Target: 5'- uCGGCCAGCGCCGUUUGUCCAACGGCUg -3' miRNA: 3'- -GCCGGUCGCGGCAAACAGGUUGCCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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