Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11285 | 3' | -59.8 | NC_003083.1 | + | 88219 | 0.66 | 0.714655 |
Target: 5'- aCGACCGG-CGCGgcUCCAaauuuuuauauUGGCcCCGUCu -3' miRNA: 3'- -GCUGGUCgGCGC--AGGU-----------GCCGuGGUAG- -5' |
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11285 | 3' | -59.8 | NC_003083.1 | + | 90989 | 0.66 | 0.714655 |
Target: 5'- uGuCCAGCCGCGcCCguacAUGGCgacACCAa- -3' miRNA: 3'- gCuGGUCGGCGCaGG----UGCCG---UGGUag -5' |
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11285 | 3' | -59.8 | NC_003083.1 | + | 4634 | 0.66 | 0.714655 |
Target: 5'- uGACCAccGCUGCGUUUgucaauCGGCACUAa- -3' miRNA: 3'- gCUGGU--CGGCGCAGGu-----GCCGUGGUag -5' |
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11285 | 3' | -59.8 | NC_003083.1 | + | 24198 | 0.66 | 0.714655 |
Target: 5'- uGGCCaAGCCGauugCCAacGCACCGUCg -3' miRNA: 3'- gCUGG-UCGGCgca-GGUgcCGUGGUAG- -5' |
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11285 | 3' | -59.8 | NC_003083.1 | + | 35738 | 0.66 | 0.70477 |
Target: 5'- uGAUguGCUuuucgGCGUCCACGuuGCCGUa -3' miRNA: 3'- gCUGguCGG-----CGCAGGUGCcgUGGUAg -5' |
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11285 | 3' | -59.8 | NC_003083.1 | + | 88067 | 0.66 | 0.687834 |
Target: 5'- gGGCCaauauaaaaauuuggAGCCGCG-CCggucguuacccucGCGGCACCGc- -3' miRNA: 3'- gCUGG---------------UCGGCGCaGG-------------UGCCGUGGUag -5' |
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11285 | 3' | -59.8 | NC_003083.1 | + | 24858 | 0.66 | 0.684831 |
Target: 5'- uGGCCGGCCGCGUUgcaaCACuuGGUAgCAa- -3' miRNA: 3'- gCUGGUCGGCGCAG----GUG--CCGUgGUag -5' |
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11285 | 3' | -59.8 | NC_003083.1 | + | 65480 | 0.66 | 0.674793 |
Target: 5'- gCGAaaaCAGCgGCGUaaGCGGUAUCAUg -3' miRNA: 3'- -GCUg--GUCGgCGCAggUGCCGUGGUAg -5' |
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11285 | 3' | -59.8 | NC_003083.1 | + | 87843 | 0.66 | 0.674793 |
Target: 5'- aCGACCAcGuCCGCGUCCGCcacuucguuuGCAUCggGUCg -3' miRNA: 3'- -GCUGGU-C-GGCGCAGGUGc---------CGUGG--UAG- -5' |
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11285 | 3' | -59.8 | NC_003083.1 | + | 40021 | 0.66 | 0.671775 |
Target: 5'- gGGCCAGCgG-GUUCaacgauuuucaacgGCGGCACCAa- -3' miRNA: 3'- gCUGGUCGgCgCAGG--------------UGCCGUGGUag -5' |
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11285 | 3' | -59.8 | NC_003083.1 | + | 32146 | 0.66 | 0.664721 |
Target: 5'- uCGGCCAGCCGuUGgacaaACGGCGCUg-- -3' miRNA: 3'- -GCUGGUCGGC-GCagg--UGCCGUGGuag -5' |
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11285 | 3' | -59.8 | NC_003083.1 | + | 89845 | 0.66 | 0.664721 |
Target: 5'- uGACU-GCCGCaUUCAUGGUgaucGCCGUCg -3' miRNA: 3'- gCUGGuCGGCGcAGGUGCCG----UGGUAG- -5' |
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11285 | 3' | -59.8 | NC_003083.1 | + | 112649 | 0.67 | 0.65159 |
Target: 5'- aCGAUguGCCGCGaauacgcaacguguUCCAgGGCAUUAg- -3' miRNA: 3'- -GCUGguCGGCGC--------------AGGUgCCGUGGUag -5' |
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11285 | 3' | -59.8 | NC_003083.1 | + | 89315 | 0.67 | 0.614134 |
Target: 5'- -uGCCGGCCGCG-CCGCaaaucucGCugGCCGUCa -3' miRNA: 3'- gcUGGUCGGCGCaGGUGc------CG--UGGUAG- -5' |
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11285 | 3' | -59.8 | NC_003083.1 | + | 1770 | 0.67 | 0.614134 |
Target: 5'- ---aCAG-CGCG-CCACGGCGCCAa- -3' miRNA: 3'- gcugGUCgGCGCaGGUGCCGUGGUag -5' |
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11285 | 3' | -59.8 | NC_003083.1 | + | 32296 | 0.68 | 0.573854 |
Target: 5'- uCGGCCAgcGCCGUuuGUCCaACGGCugGCCGa- -3' miRNA: 3'- -GCUGGU--CGGCG--CAGG-UGCCG--UGGUag -5' |
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11285 | 3' | -59.8 | NC_003083.1 | + | 38209 | 0.69 | 0.534216 |
Target: 5'- aGAaCAcGCgaCGUG-CCGCGGCACCAUCa -3' miRNA: 3'- gCUgGU-CG--GCGCaGGUGCCGUGGUAG- -5' |
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11285 | 3' | -59.8 | NC_003083.1 | + | 62614 | 0.69 | 0.49559 |
Target: 5'- aCGACaC-GCCGgGggCCACGaGCGCCAUUa -3' miRNA: 3'- -GCUG-GuCGGCgCa-GGUGC-CGUGGUAG- -5' |
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11285 | 3' | -59.8 | NC_003083.1 | + | 51775 | 1.1 | 0.000899 |
Target: 5'- gCGACCAGCCGCGUCCACGGCACCAUCg -3' miRNA: 3'- -GCUGGUCGGCGCAGGUGCCGUGGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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