Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11285 | 5' | -53 | NC_003083.1 | + | 109230 | 0.66 | 0.918214 |
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Target: 5'- cAAGGUUGCacaGCGCGUUUguaCCGCAauuUGCUAu -3' miRNA: 3'- -UUCUAGCG---UGCGCAAA---GGUGU---GCGAU- -5' |
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| 11285 | 5' | -53 | NC_003083.1 | + | 62667 | 0.66 | 0.918214 |
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Target: 5'- cAGAUCGC-CGUGUUcgUCugACGCa- -3' miRNA: 3'- uUCUAGCGuGCGCAAa-GGugUGCGau -5' |
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| 11285 | 5' | -53 | NC_003083.1 | + | 115987 | 0.67 | 0.892031 |
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Target: 5'- ----cCGCACgaGCGUUUCCGCuCGCUu -3' miRNA: 3'- uucuaGCGUG--CGCAAAGGUGuGCGAu -5' |
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| 11285 | 5' | -53 | NC_003083.1 | + | 70783 | 0.68 | 0.877399 |
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Target: 5'- -uGGUgGCGCGCGUaaaCCugguCACGCUAu -3' miRNA: 3'- uuCUAgCGUGCGCAaa-GGu---GUGCGAU- -5' |
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| 11285 | 5' | -53 | NC_003083.1 | + | 37644 | 0.68 | 0.845257 |
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Target: 5'- aAGGAUCGCGauugcgcuCGCGUUUCCA-ACGUUc -3' miRNA: 3'- -UUCUAGCGU--------GCGCAAAGGUgUGCGAu -5' |
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| 11285 | 5' | -53 | NC_003083.1 | + | 58820 | 0.69 | 0.836663 |
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Target: 5'- --uAUCGCACGgGUUgaUCgGCACGCa- -3' miRNA: 3'- uucUAGCGUGCgCAA--AGgUGUGCGau -5' |
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| 11285 | 5' | -53 | NC_003083.1 | + | 54757 | 0.7 | 0.740877 |
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Target: 5'- uAGGAUUGCGCGCGUaaaUUUCuCGCGCc- -3' miRNA: 3'- -UUCUAGCGUGCGCA---AAGGuGUGCGau -5' |
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| 11285 | 5' | -53 | NC_003083.1 | + | 51287 | 0.71 | 0.720106 |
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Target: 5'- cGAGcgCGCGCGCGUUUguuugaCgGCGCGUUAg -3' miRNA: 3'- -UUCuaGCGUGCGCAAA------GgUGUGCGAU- -5' |
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| 11285 | 5' | -53 | NC_003083.1 | + | 51740 | 0.92 | 0.043643 |
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Target: 5'- cAAGAUCGCACGCGUUUCCACA-GCUAc -3' miRNA: 3'- -UUCUAGCGUGCGCAAAGGUGUgCGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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