Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11286 | 3' | -50.2 | NC_003083.1 | + | 18333 | 0.66 | 0.980918 |
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Target: 5'- --gCGUUgacaacagcGCCGGCGUACaaGCAAGAc -3' miRNA: 3'- uaaGCAA---------CGGUCGCAUGcgCGUUUUu -5' |
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| 11286 | 3' | -50.2 | NC_003083.1 | + | 71115 | 0.66 | 0.975934 |
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Target: 5'- -aUUGUUGCCAGU---CGCGCAc--- -3' miRNA: 3'- uaAGCAACGGUCGcauGCGCGUuuuu -5' |
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| 11286 | 3' | -50.2 | NC_003083.1 | + | 28318 | 0.67 | 0.970059 |
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Target: 5'- ----uUUGCCggaAGCGUgucGCGCGCAAGAc -3' miRNA: 3'- uaagcAACGG---UCGCA---UGCGCGUUUUu -5' |
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| 11286 | 3' | -50.2 | NC_003083.1 | + | 87491 | 0.67 | 0.970059 |
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Target: 5'- -aUCGUcuaaacguacuUGaCCGGCagcgcacgGUGCGCGCGAAAAa -3' miRNA: 3'- uaAGCA-----------AC-GGUCG--------CAUGCGCGUUUUU- -5' |
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| 11286 | 3' | -50.2 | NC_003083.1 | + | 71278 | 0.68 | 0.95537 |
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Target: 5'- -aUCGUcaCCAGUGUGCGCGUg---- -3' miRNA: 3'- uaAGCAacGGUCGCAUGCGCGuuuuu -5' |
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| 11286 | 3' | -50.2 | NC_003083.1 | + | 38381 | 0.68 | 0.951045 |
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Target: 5'- --aCGUUGCgAGCGU-CGUGUGGAAu -3' miRNA: 3'- uaaGCAACGgUCGCAuGCGCGUUUUu -5' |
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| 11286 | 3' | -50.2 | NC_003083.1 | + | 66551 | 0.69 | 0.906464 |
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Target: 5'- cUUCGUgcacGCUAGUGUACaCGCGAAAc -3' miRNA: 3'- uAAGCAa---CGGUCGCAUGcGCGUUUUu -5' |
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| 11286 | 3' | -50.2 | NC_003083.1 | + | 60599 | 0.74 | 0.715414 |
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Target: 5'- --cCGUUGCCcGUGUACGCGUAc--- -3' miRNA: 3'- uaaGCAACGGuCGCAUGCGCGUuuuu -5' |
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| 11286 | 3' | -50.2 | NC_003083.1 | + | 42435 | 0.74 | 0.693663 |
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Target: 5'- -aUCGUUGCaCAGCGUGCaGCGguGGc- -3' miRNA: 3'- uaAGCAACG-GUCGCAUG-CGCguUUuu -5' |
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| 11286 | 3' | -50.2 | NC_003083.1 | + | 56955 | 1.04 | 0.01194 |
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Target: 5'- aAUUCGUUGCCAGCGUACGCGCAAAAAu -3' miRNA: 3'- -UAAGCAACGGUCGCAUGCGCGUUUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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