Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11288 | 5' | -53.3 | NC_003083.1 | + | 72997 | 0.66 | 0.959028 |
Target: 5'- aUGGuCUaccGCAGACGUCGAUCc-GCAGa -3' miRNA: 3'- gACC-GA---UGUUUGCAGCUGGacCGUCc -5' |
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11288 | 5' | -53.3 | NC_003083.1 | + | 35240 | 0.67 | 0.921127 |
Target: 5'- --uGCUGCAAuUGUCGugCUGGCu-- -3' miRNA: 3'- gacCGAUGUUuGCAGCugGACCGucc -5' |
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11288 | 5' | -53.3 | NC_003083.1 | + | 1974 | 0.68 | 0.902756 |
Target: 5'- -aGGCUGCGgcGACGcCGGCgUgcaccgacgacGGCAGGu -3' miRNA: 3'- gaCCGAUGU--UUGCaGCUGgA-----------CCGUCC- -5' |
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11288 | 5' | -53.3 | NC_003083.1 | + | 72765 | 0.69 | 0.85706 |
Target: 5'- -cGGCUGCGGcuucuucgguacguGCG-CGGCCUGcCAGGu -3' miRNA: 3'- gaCCGAUGUU--------------UGCaGCUGGACcGUCC- -5' |
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11288 | 5' | -53.3 | NC_003083.1 | + | 114238 | 0.69 | 0.843197 |
Target: 5'- -gGGCUGCGgcgcugcaccGGCGUCGugUUGuCAGGu -3' miRNA: 3'- gaCCGAUGU----------UUGCAGCugGACcGUCC- -5' |
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11288 | 5' | -53.3 | NC_003083.1 | + | 96860 | 0.72 | 0.679502 |
Target: 5'- -gGGCUACAuuuAGCGUauGCUUGGUGGGg -3' miRNA: 3'- gaCCGAUGU---UUGCAgcUGGACCGUCC- -5' |
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11288 | 5' | -53.3 | NC_003083.1 | + | 76167 | 0.73 | 0.658516 |
Target: 5'- gCUGGCaAC-AGCGUCuGuuCUGGCAGGc -3' miRNA: 3'- -GACCGaUGuUUGCAG-CugGACCGUCC- -5' |
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11288 | 5' | -53.3 | NC_003083.1 | + | 72937 | 1.1 | 0.003668 |
Target: 5'- uCUGGCUACAAACGUCGACCUGGCAGGc -3' miRNA: 3'- -GACCGAUGUUUGCAGCUGGACCGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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