miRNA: 3'- gUCGaaGAaa--CGCUUG-GCGGGUGUgc -5'
miRNA: 3'- guCGAAGAaACGCuug-GCGGGUGUGc -5'
miRNA: 3'- gUCGaagaaACGcUugGCGGGUGUGC- -5'
miRNA: 3'- guCGAAGAAACGCU--UGGCGgGUGUGC- -5'
miRNA: 3'- -GUCGAAGAAACGCUUGGCGGGUGUGC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11291 | 3' | -53.1 | NC_003083.1 | + | 74220 | 0.67 | 0.931684 |
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Target: 5'- gAGUcgCUgcaaGCGAACgCGCCCACAa- -3' miRNA: 3'- gUCGaaGAaa--CGCUUG-GCGGGUGUgc -5' |
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| 11291 | 3' | -53.1 | NC_003083.1 | + | 68925 | 0.67 | 0.914599 |
|
Target: 5'- -cGUUUUUgUGCGuaaaCGCCCACACa -3' miRNA: 3'- guCGAAGAaACGCuug-GCGGGUGUGc -5' |
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| 11291 | 3' | -53.1 | NC_003083.1 | + | 44370 | 0.67 | 0.914599 |
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Target: 5'- gGGCgcc--UGCaGugCGCCCGCACGg -3' miRNA: 3'- gUCGaagaaACGcUugGCGGGUGUGC- -5' |
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| 11291 | 3' | -53.1 | NC_003083.1 | + | 24777 | 0.73 | 0.622554 |
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Target: 5'- -uGCUUUUUUGUGAuuGCCGCgUGCGCGa -3' miRNA: 3'- guCGAAGAAACGCU--UGGCGgGUGUGC- -5' |
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| 11291 | 3' | -53.1 | NC_003083.1 | + | 90017 | 1.09 | 0.003751 |
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Target: 5'- gCAGCUUCUUUGCGAACCGCCCACACGa -3' miRNA: 3'- -GUCGAAGAAACGCUUGGCGGGUGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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