miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
11292 5' -50.2 NC_003083.1 + 16648 0.66 0.983981
Target:  5'- uAGGCACugugaaagAAGCGAcccGUUucuucgacaaacUCGCGCACUg -3'
miRNA:   3'- -UCUGUG--------UUCGCUu--CAA------------AGCGCGUGAg -5'
11292 5' -50.2 NC_003083.1 + 115025 0.67 0.97167
Target:  5'- cAGAUAUAGGCGAGGcgcaGaCGCACgUCa -3'
miRNA:   3'- -UCUGUGUUCGCUUCaaagC-GCGUG-AG- -5'
11292 5' -50.2 NC_003083.1 + 51844 0.68 0.968558
Target:  5'- uAGGCAaauuAGCGcuaauuGAGUUUCGCGCAa-- -3'
miRNA:   3'- -UCUGUgu--UCGC------UUCAAAGCGCGUgag -5'
11292 5' -50.2 NC_003083.1 + 70680 0.68 0.954943
Target:  5'- cGACACGGGCGuguacucgacggCGUGCACUUc -3'
miRNA:   3'- uCUGUGUUCGCuucaaa------GCGCGUGAG- -5'
11292 5' -50.2 NC_003083.1 + 81012 0.73 0.807437
Target:  5'- uAGGCGCGAGUGA---UUUGCGCGCa- -3'
miRNA:   3'- -UCUGUGUUCGCUucaAAGCGCGUGag -5'
11292 5' -50.2 NC_003083.1 + 100726 1.09 0.006771
Target:  5'- cAGACACAAGCGAAGUUUCGCGCACUCc -3'
miRNA:   3'- -UCUGUGUUCGCUUCAAAGCGCGUGAG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.