Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11292 | 5' | -50.2 | NC_003083.1 | + | 16648 | 0.66 | 0.983981 |
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Target: 5'- uAGGCACugugaaagAAGCGAcccGUUucuucgacaaacUCGCGCACUg -3' miRNA: 3'- -UCUGUG--------UUCGCUu--CAA------------AGCGCGUGAg -5' |
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| 11292 | 5' | -50.2 | NC_003083.1 | + | 115025 | 0.67 | 0.97167 |
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Target: 5'- cAGAUAUAGGCGAGGcgcaGaCGCACgUCa -3' miRNA: 3'- -UCUGUGUUCGCUUCaaagC-GCGUG-AG- -5' |
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| 11292 | 5' | -50.2 | NC_003083.1 | + | 51844 | 0.68 | 0.968558 |
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Target: 5'- uAGGCAaauuAGCGcuaauuGAGUUUCGCGCAa-- -3' miRNA: 3'- -UCUGUgu--UCGC------UUCAAAGCGCGUgag -5' |
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| 11292 | 5' | -50.2 | NC_003083.1 | + | 70680 | 0.68 | 0.954943 |
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Target: 5'- cGACACGGGCGuguacucgacggCGUGCACUUc -3' miRNA: 3'- uCUGUGUUCGCuucaaa------GCGCGUGAG- -5' |
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| 11292 | 5' | -50.2 | NC_003083.1 | + | 81012 | 0.73 | 0.807437 |
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Target: 5'- uAGGCGCGAGUGA---UUUGCGCGCa- -3' miRNA: 3'- -UCUGUGUUCGCUucaAAGCGCGUGag -5' |
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| 11292 | 5' | -50.2 | NC_003083.1 | + | 100726 | 1.09 | 0.006771 |
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Target: 5'- cAGACACAAGCGAAGUUUCGCGCACUCc -3' miRNA: 3'- -UCUGUGUUCGCUUCAAAGCGCGUGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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