Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11293 | 5' | -51.7 | NC_003083.1 | + | 94806 | 0.66 | 0.972291 |
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Target: 5'- cGCGauuUUUCCGGuCGCGGCGUUUggguGCGCGg -3' miRNA: 3'- -CGU---GAAGGCC-GUGUUGUAGGu---UGCGU- -5' |
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| 11293 | 5' | -51.7 | NC_003083.1 | + | 97752 | 0.67 | 0.958459 |
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Target: 5'- aGCAgUUCguuuugaUGGCcCAGCuuuUCCAACGCGu -3' miRNA: 3'- -CGUgAAG-------GCCGuGUUGu--AGGUUGCGU- -5' |
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| 11293 | 5' | -51.7 | NC_003083.1 | + | 98826 | 0.72 | 0.77515 |
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Target: 5'- -aACUcaUCCGGCACGu--UCCAugGCGu -3' miRNA: 3'- cgUGA--AGGCCGUGUuguAGGUugCGU- -5' |
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| 11293 | 5' | -51.7 | NC_003083.1 | + | 100901 | 0.69 | 0.890041 |
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Target: 5'- cGCACUUCUGaauuguuguaaaacaGCACGcACGaaUCUAGCGCAu -3' miRNA: 3'- -CGUGAAGGC---------------CGUGU-UGU--AGGUUGCGU- -5' |
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| 11293 | 5' | -51.7 | NC_003083.1 | + | 102724 | 0.67 | 0.946206 |
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Target: 5'- uGCACUUCguuuuCGGCACcuucacGCAUCgCAgcuGCGCGa -3' miRNA: 3'- -CGUGAAG-----GCCGUGu-----UGUAG-GU---UGCGU- -5' |
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| 11293 | 5' | -51.7 | NC_003083.1 | + | 105201 | 1.11 | 0.00395 |
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Target: 5'- aGCACUUCCGGCACAACAUCCAACGCAc -3' miRNA: 3'- -CGUGAAGGCCGUGUUGUAGGUUGCGU- -5' |
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| 11293 | 5' | -51.7 | NC_003083.1 | + | 106226 | 0.7 | 0.856479 |
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Target: 5'- --uUUUCCGcGCACAACAagCAAUGCGa -3' miRNA: 3'- cguGAAGGC-CGUGUUGUagGUUGCGU- -5' |
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| 11293 | 5' | -51.7 | NC_003083.1 | + | 107818 | 0.66 | 0.972291 |
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Target: 5'- cCGCcaCUGGCGCAACcacgagcaCCGACGCGu -3' miRNA: 3'- cGUGaaGGCCGUGUUGua------GGUUGCGU- -5' |
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| 11293 | 5' | -51.7 | NC_003083.1 | + | 114211 | 0.69 | 0.901068 |
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Target: 5'- gGCGCUggUUCGGCugGugaAUAUCCAGgGCu -3' miRNA: 3'- -CGUGA--AGGCCGugU---UGUAGGUUgCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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