Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11294 | 5' | -45.4 | NC_003083.1 | + | 45837 | 0.67 | 0.999776 |
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Target: 5'- aCGCGUCccguUCGUUcgaUUCUGUGGugGGGu -3' miRNA: 3'- -GUGCGGu---AGCAA---AAGACAUUugUCUu -5' |
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| 11294 | 5' | -45.4 | NC_003083.1 | + | 55087 | 0.67 | 0.999629 |
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Target: 5'- gGCGCCAUUGUgUUUUGUAuaauguUAGGAa -3' miRNA: 3'- gUGCGGUAGCAaAAGACAUuu----GUCUU- -5' |
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| 11294 | 5' | -45.4 | NC_003083.1 | + | 32332 | 0.67 | 0.999601 |
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Target: 5'- aCGCGUUGUUGUUUuaacccgcgacacgUCUGUAAACAcGAAu -3' miRNA: 3'- -GUGCGGUAGCAAA--------------AGACAUUUGU-CUU- -5' |
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| 11294 | 5' | -45.4 | NC_003083.1 | + | 3492 | 0.73 | 0.970852 |
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Target: 5'- gCACGCCGUC---UUCUGUAAACAc-- -3' miRNA: 3'- -GUGCGGUAGcaaAAGACAUUUGUcuu -5' |
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| 11294 | 5' | -45.4 | NC_003083.1 | + | 16695 | 0.74 | 0.960131 |
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Target: 5'- uCACGCCGUCGUccgUCcaaugcaacGUGAACGGAAa -3' miRNA: 3'- -GUGCGGUAGCAaa-AGa--------CAUUUGUCUU- -5' |
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| 11294 | 5' | -45.4 | NC_003083.1 | + | 108591 | 1.07 | 0.027166 |
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Target: 5'- aCACGCCAUCGUUUUCUGUAAACAGAAu -3' miRNA: 3'- -GUGCGGUAGCAAAAGACAUUUGUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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