Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11297 | 5' | -50 | NC_003083.1 | + | 106853 | 0.66 | 0.991383 |
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Target: 5'- ---gUGCguAUGUGCGCCGcgccuaggUUGGa -3' miRNA: 3'- guuaACGguUGCACGCGGCua------AACCa -5' |
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| 11297 | 5' | -50 | NC_003083.1 | + | 1962 | 0.66 | 0.990096 |
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Target: 5'- ----cGCCGGCGUGCaCCGAcgacggcaggUGGUc -3' miRNA: 3'- guuaaCGGUUGCACGcGGCUaa--------ACCA- -5' |
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| 11297 | 5' | -50 | NC_003083.1 | + | 2161 | 0.67 | 0.978953 |
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Target: 5'- -uAUUGCUggagcagucaAACGgGCGCCGggUUGGa -3' miRNA: 3'- guUAACGG----------UUGCaCGCGGCuaAACCa -5' |
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| 11297 | 5' | -50 | NC_003083.1 | + | 96696 | 0.68 | 0.967553 |
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Target: 5'- ---cUGCUAACccgGCGCCGGUcgGGg -3' miRNA: 3'- guuaACGGUUGca-CGCGGCUAaaCCa -5' |
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| 11297 | 5' | -50 | NC_003083.1 | + | 27835 | 0.69 | 0.93822 |
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Target: 5'- ----gGCCAGCGUGCGUgG--UUGGg -3' miRNA: 3'- guuaaCGGUUGCACGCGgCuaAACCa -5' |
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| 11297 | 5' | -50 | NC_003083.1 | + | 2599 | 0.71 | 0.888636 |
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Target: 5'- --uUUGCCuaaaAGCGUGCGCUGAcg-GGUc -3' miRNA: 3'- guuAACGG----UUGCACGCGGCUaaaCCA- -5' |
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| 11297 | 5' | -50 | NC_003083.1 | + | 24767 | 0.71 | 0.873595 |
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Target: 5'- uGAUUGCC-GCGUGCG-CGAU-UGGUu -3' miRNA: 3'- gUUAACGGuUGCACGCgGCUAaACCA- -5' |
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| 11297 | 5' | -50 | NC_003083.1 | + | 3104 | 1.08 | 0.009207 |
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Target: 5'- aCAAUUGCCAACGUGCGCCGAUUUGGUu -3' miRNA: 3'- -GUUAACGGUUGCACGCGGCUAAACCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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