Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
113 | 3' | -57 | AC_000006.1 | + | 8321 | 0.67 | 0.431062 |
Target: 5'- aCGCGcucccgGCGGcagcGGcgGUUCcgGCCCCGCg -3' miRNA: 3'- -GUGCaa----CGCCc---CCuaCAAG--UGGGGCG- -5' |
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113 | 3' | -57 | AC_000006.1 | + | 4609 | 0.68 | 0.393147 |
Target: 5'- ----cUGCGaGGGGAacUUCGCCCCGg -3' miRNA: 3'- gugcaACGC-CCCCUacAAGUGGGGCg -5' |
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113 | 3' | -57 | AC_000006.1 | + | 25086 | 0.7 | 0.316151 |
Target: 5'- aGCG--GCGGGcgaaacGAUGUUCcCCCCGCa -3' miRNA: 3'- gUGCaaCGCCCc-----CUACAAGuGGGGCG- -5' |
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113 | 3' | -57 | AC_000006.1 | + | 15874 | 0.7 | 0.308333 |
Target: 5'- aCGCGUguUGCGGcccggcacggcGGuGGUGUUCACgCCCGg -3' miRNA: 3'- -GUGCA--ACGCC-----------CC-CUACAAGUG-GGGCg -5' |
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113 | 3' | -57 | AC_000006.1 | + | 10508 | 0.7 | 0.293142 |
Target: 5'- gACGgccGCGGcgagcGGGAcuUGgUCACCCCGCc -3' miRNA: 3'- gUGCaa-CGCC-----CCCU--ACaAGUGGGGCG- -5' |
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113 | 3' | -57 | AC_000006.1 | + | 25127 | 1 | 0.001925 |
Target: 5'- aCACGUU-CGGGGGAUGUUCACCCCGCg -3' miRNA: 3'- -GUGCAAcGCCCCCUACAAGUGGGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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