Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
113 | 5' | -56 | AC_000006.1 | + | 5750 | 0.66 | 0.534958 |
Target: 5'- cGGUGGAAaaGGUGgaCCCCCUGguGg -3' miRNA: 3'- cCCGCUUUg-CUACaaGGGGGGCguCa -5' |
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113 | 5' | -56 | AC_000006.1 | + | 5129 | 0.68 | 0.440824 |
Target: 5'- cGGUGAAGgGGUGggCCCCUgGCuGg -3' miRNA: 3'- cCCGCUUUgCUACaaGGGGGgCGuCa -5' |
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113 | 5' | -56 | AC_000006.1 | + | 15263 | 0.69 | 0.365584 |
Target: 5'- cGGCGGcGGCGGUGgcagUCCgCCCGCGc- -3' miRNA: 3'- cCCGCU-UUGCUACa---AGGgGGGCGUca -5' |
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113 | 5' | -56 | AC_000006.1 | + | 24500 | 0.69 | 0.365584 |
Target: 5'- -aGCGGAGCGGUGga-CUCCUGCGGa -3' miRNA: 3'- ccCGCUUUGCUACaagGGGGGCGUCa -5' |
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113 | 5' | -56 | AC_000006.1 | + | 6541 | 0.71 | 0.302752 |
Target: 5'- uGGGCuccGAGAagaugcCGAUGUUggugggauaacagcgCCCCCCGCGGa -3' miRNA: 3'- -CCCG---CUUU------GCUACAA---------------GGGGGGCGUCa -5' |
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113 | 5' | -56 | AC_000006.1 | + | 8330 | 0.74 | 0.180575 |
Target: 5'- cGGCGGcAGCGGcgGUUCCggCCCCGCGGg -3' miRNA: 3'- cCCGCU-UUGCUa-CAAGG--GGGGCGUCa -5' |
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113 | 5' | -56 | AC_000006.1 | + | 4547 | 0.83 | 0.039741 |
Target: 5'- cGGGCcccGggGCGAaGUUCCCCUCGCAGa -3' miRNA: 3'- -CCCG---CuuUGCUaCAAGGGGGGCGUCa -5' |
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113 | 5' | -56 | AC_000006.1 | + | 25081 | 1.09 | 0.000494 |
Target: 5'- cGGGCGAAACGAUGUUCCCCCCGCAGUc -3' miRNA: 3'- -CCCGCUUUGCUACAAGGGGGGCGUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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