Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11300 | 5' | -49.7 | NC_003083.1 | + | 89252 | 0.66 | 0.990967 |
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Target: 5'- uUAAAAacauACGCGCGUUGAUCGCa----- -3' miRNA: 3'- -AUUUU----UGCGCGCGAUUGGCGcucaua -5' |
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| 11300 | 5' | -49.7 | NC_003083.1 | + | 54501 | 0.66 | 0.98644 |
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Target: 5'- ------gGUGCGCUGGCCGUGuuuagccuAGUGUa -3' miRNA: 3'- auuuuugCGCGCGAUUGGCGC--------UCAUA- -5' |
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| 11300 | 5' | -49.7 | NC_003083.1 | + | 2594 | 0.67 | 0.980347 |
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Target: 5'- cUAAAAGCGUGCGCUGACggguCGC-AGa-- -3' miRNA: 3'- -AUUUUUGCGCGCGAUUG----GCGcUCaua -5' |
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| 11300 | 5' | -49.7 | NC_003083.1 | + | 87917 | 0.68 | 0.958553 |
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Target: 5'- -cAGGAUGCgGCGUUAGCaGCGGGUAc -3' miRNA: 3'- auUUUUGCG-CGCGAUUGgCGCUCAUa -5' |
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| 11300 | 5' | -49.7 | NC_003083.1 | + | 116962 | 0.69 | 0.947337 |
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Target: 5'- aAAuuACGCGCGCUcuuuugacugguacGCUGUGGGUGUg -3' miRNA: 3'- aUUuuUGCGCGCGAu-------------UGGCGCUCAUA- -5' |
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| 11300 | 5' | -49.7 | NC_003083.1 | + | 35825 | 0.69 | 0.94545 |
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Target: 5'- -cAAAACGaCGCGCUuuuuGCaCGCGAcGUGUa -3' miRNA: 3'- auUUUUGC-GCGCGAu---UG-GCGCU-CAUA- -5' |
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| 11300 | 5' | -49.7 | NC_003083.1 | + | 18399 | 0.7 | 0.924141 |
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Target: 5'- --cAAGCGCGCGCUAAgCGCa----- -3' miRNA: 3'- auuUUUGCGCGCGAUUgGCGcucaua -5' |
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| 11300 | 5' | -49.7 | NC_003083.1 | + | 71801 | 0.72 | 0.851755 |
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Target: 5'- cAAuAGCGCGgGUUGGCCGCGAuGUc- -3' miRNA: 3'- aUUuUUGCGCgCGAUUGGCGCU-CAua -5' |
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| 11300 | 5' | -49.7 | NC_003083.1 | + | 88244 | 0.73 | 0.796816 |
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Target: 5'- ---cAGCGgGCGCgguGCCGCGAGg-- -3' miRNA: 3'- auuuUUGCgCGCGau-UGGCGCUCaua -5' |
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| 11300 | 5' | -49.7 | NC_003083.1 | + | 22305 | 1.04 | 0.015383 |
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Target: 5'- uUAAAAACGCGCGCUAACCGCGAGUAUu -3' miRNA: 3'- -AUUUUUGCGCGCGAUUGGCGCUCAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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