Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11301 | 3' | -51.7 | NC_003083.1 | + | 84598 | 0.66 | 0.975492 |
Target: 5'- -----aGACGCACG-CGUCGGCGg-- -3' miRNA: 3'- cucauaCUGUGUGCgGCAGCUGCaua -5' |
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11301 | 3' | -51.7 | NC_003083.1 | + | 74424 | 0.67 | 0.964516 |
Target: 5'- ---gAUGACGCugacacaccgccgcgGCGUCGUCGGCGUu- -3' miRNA: 3'- cucaUACUGUG---------------UGCGGCAGCUGCAua -5' |
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11301 | 3' | -51.7 | NC_003083.1 | + | 23119 | 0.68 | 0.926643 |
Target: 5'- uAGUAUGccgGCgGCGCGCUGUUGGCGUu- -3' miRNA: 3'- cUCAUAC---UG-UGUGCGGCAGCUGCAua -5' |
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11301 | 3' | -51.7 | NC_003083.1 | + | 4496 | 0.76 | 0.535259 |
Target: 5'- --aUGUGACACGCGUCGUUGACGc-- -3' miRNA: 3'- cucAUACUGUGUGCGGCAGCUGCaua -5' |
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11301 | 3' | -51.7 | NC_003083.1 | + | 24006 | 1.06 | 0.00901 |
Target: 5'- cGAGUAUGACACACGCCGUCGACGUAUc -3' miRNA: 3'- -CUCAUACUGUGUGCGGCAGCUGCAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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