Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11301 | 5' | -56.5 | NC_003083.1 | + | 64506 | 0.66 | 0.83367 |
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Target: 5'- gGCGUUgGCgaagggguuggCGAUGGUggagGCGUUGGCGAa -3' miRNA: 3'- -UGUAGgCG-----------GCUGCCA----CGCAACCGUUa -5' |
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| 11301 | 5' | -56.5 | NC_003083.1 | + | 8239 | 0.66 | 0.825086 |
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Target: 5'- cACAauUCCGuuGACGGacGCGUuuaauUGGCGc- -3' miRNA: 3'- -UGU--AGGCggCUGCCa-CGCA-----ACCGUua -5' |
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| 11301 | 5' | -56.5 | NC_003083.1 | + | 50847 | 0.66 | 0.81632 |
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Target: 5'- cGCGUuuagUCGCCauGugGGaaacugUGCGUUGGCAAUu -3' miRNA: 3'- -UGUA----GGCGG--CugCC------ACGCAACCGUUA- -5' |
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| 11301 | 5' | -56.5 | NC_003083.1 | + | 43570 | 0.67 | 0.779591 |
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Target: 5'- aACcUUCGCCGACGGgaucaaUGCGUUGcCAAc -3' miRNA: 3'- -UGuAGGCGGCUGCC------ACGCAACcGUUa -5' |
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| 11301 | 5' | -56.5 | NC_003083.1 | + | 84733 | 0.68 | 0.710377 |
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Target: 5'- ---aCCGCCGACGcGUGCGUcuGCGc- -3' miRNA: 3'- uguaGGCGGCUGC-CACGCAacCGUua -5' |
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| 11301 | 5' | -56.5 | NC_003083.1 | + | 112096 | 0.68 | 0.689829 |
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Target: 5'- cACGUCUGCuUGAUucauguuagGGUcGCGUUGGCAAg -3' miRNA: 3'- -UGUAGGCG-GCUG---------CCA-CGCAACCGUUa -5' |
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| 11301 | 5' | -56.5 | NC_003083.1 | + | 7816 | 0.7 | 0.575161 |
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Target: 5'- cACGUuuGUCGGCGGUaccgccgggGCGUUGaGCGAa -3' miRNA: 3'- -UGUAggCGGCUGCCA---------CGCAAC-CGUUa -5' |
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| 11301 | 5' | -56.5 | NC_003083.1 | + | 51624 | 0.75 | 0.312784 |
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Target: 5'- cGCGUuuGU--ACGGUGCGUUGGCAAUu -3' miRNA: 3'- -UGUAggCGgcUGCCACGCAACCGUUA- -5' |
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| 11301 | 5' | -56.5 | NC_003083.1 | + | 107552 | 0.77 | 0.264619 |
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Target: 5'- -aGUUCGCCGcCGGUGCGaUUGGCGGg -3' miRNA: 3'- ugUAGGCGGCuGCCACGC-AACCGUUa -5' |
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| 11301 | 5' | -56.5 | NC_003083.1 | + | 24043 | 1.05 | 0.003328 |
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Target: 5'- gACAUCCGCCGACGGUGCGUUGGCAAUc -3' miRNA: 3'- -UGUAGGCGGCUGCCACGCAACCGUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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