Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11302 | 5' | -57.7 | NC_003083.1 | + | 23387 | 0.66 | 0.788574 |
Target: 5'- cGCGCGCcacacuuuuucgUCAuugCGcGCCGCGUCu- -3' miRNA: 3'- aCGCGCG------------AGUuaaGCcCGGCGCAGug -5' |
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11302 | 5' | -57.7 | NC_003083.1 | + | 28477 | 0.66 | 0.788574 |
Target: 5'- cGCGCGCgugCAuugccccCGGGCCGC--CAUa -3' miRNA: 3'- aCGCGCGa--GUuaa----GCCCGGCGcaGUG- -5' |
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11302 | 5' | -57.7 | NC_003083.1 | + | 11927 | 0.66 | 0.779268 |
Target: 5'- aGgGCGCUCAucaacaaCGGGUacgGCGUCGu -3' miRNA: 3'- aCgCGCGAGUuaa----GCCCGg--CGCAGUg -5' |
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11302 | 5' | -57.7 | NC_003083.1 | + | 56462 | 0.66 | 0.769828 |
Target: 5'- gGC-CGCuaaugccaUCAAUUCGccGGCCGCG-CACu -3' miRNA: 3'- aCGcGCG--------AGUUAAGC--CCGGCGCaGUG- -5' |
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11302 | 5' | -57.7 | NC_003083.1 | + | 55628 | 0.67 | 0.730908 |
Target: 5'- aGCGCGCauUCAA-UCGGcGuuGCGUUuugaGCg -3' miRNA: 3'- aCGCGCG--AGUUaAGCC-CggCGCAG----UG- -5' |
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11302 | 5' | -57.7 | NC_003083.1 | + | 74091 | 0.67 | 0.730908 |
Target: 5'- uUGCaGCGaCUCAAccagcuugCGGGCCGCGa--- -3' miRNA: 3'- -ACG-CGC-GAGUUaa------GCCCGGCGCagug -5' |
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11302 | 5' | -57.7 | NC_003083.1 | + | 56963 | 0.67 | 0.710886 |
Target: 5'- -uUGUGCUCAAUUCGuuGCCaGCGUaCGCg -3' miRNA: 3'- acGCGCGAGUUAAGCc-CGG-CGCA-GUG- -5' |
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11302 | 5' | -57.7 | NC_003083.1 | + | 24886 | 0.68 | 0.638107 |
Target: 5'- cGCGCGUaaaagCAcGUUCGaagauuauuggccGGCCGCGUUGCa -3' miRNA: 3'- aCGCGCGa----GU-UAAGC-------------CCGGCGCAGUG- -5' |
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11302 | 5' | -57.7 | NC_003083.1 | + | 39561 | 0.7 | 0.556914 |
Target: 5'- aGUGCGCcauUCGAccaaguUUUGGGuuGCGUUACc -3' miRNA: 3'- aCGCGCG---AGUU------AAGCCCggCGCAGUG- -5' |
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11302 | 5' | -57.7 | NC_003083.1 | + | 27957 | 1.1 | 0.001197 |
Target: 5'- gUGCGCGCUCAAUUCGGGCCGCGUCACg -3' miRNA: 3'- -ACGCGCGAGUUAAGCCCGGCGCAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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