Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11306 | 3' | -51.7 | NC_003083.1 | + | 64496 | 0.68 | 0.927539 |
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Target: 5'- uCGCcAACGCCGucgccAACACuauggCCAACACCUu -3' miRNA: 3'- -GUGuUUGCGGC-----UUGUG-----GGUUGUGGAc -5' |
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| 11306 | 3' | -51.7 | NC_003083.1 | + | 62609 | 0.68 | 0.927538 |
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Target: 5'- cCGCGAcgacACGCCGGGgGCCacgAGCGCCa- -3' miRNA: 3'- -GUGUU----UGCGGCUUgUGGg--UUGUGGac -5' |
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| 11306 | 3' | -51.7 | NC_003083.1 | + | 99179 | 0.68 | 0.921857 |
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Target: 5'- aGCAAGCGCaGcGCGCCCuAUACCg- -3' miRNA: 3'- gUGUUUGCGgCuUGUGGGuUGUGGac -5' |
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| 11306 | 3' | -51.7 | NC_003083.1 | + | 72975 | 0.69 | 0.903242 |
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Target: 5'- cCGCAGACgGCaCGuACACCCGucgACGCCg- -3' miRNA: 3'- -GUGUUUG-CG-GCuUGUGGGU---UGUGGac -5' |
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| 11306 | 3' | -51.7 | NC_003083.1 | + | 54317 | 0.69 | 0.899239 |
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Target: 5'- aGCAAGCGCUGGACGCaauuuuaaacaaaAACACCa- -3' miRNA: 3'- gUGUUUGCGGCUUGUGgg-----------UUGUGGac -5' |
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| 11306 | 3' | -51.7 | NC_003083.1 | + | 19892 | 0.7 | 0.882319 |
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Target: 5'- uUACAGACGCCGcACAUuuGGCACa-- -3' miRNA: 3'- -GUGUUUGCGGCuUGUGggUUGUGgac -5' |
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| 11306 | 3' | -51.7 | NC_003083.1 | + | 114383 | 0.73 | 0.717895 |
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Target: 5'- aCAC-GACGCCGGugcaGCGCCgCAGC-CCUGg -3' miRNA: 3'- -GUGuUUGCGGCU----UGUGG-GUUGuGGAC- -5' |
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| 11306 | 3' | -51.7 | NC_003083.1 | + | 7981 | 0.73 | 0.696913 |
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Target: 5'- gUACAAACGCCauuCGCUCAACGCCc- -3' miRNA: 3'- -GUGUUUGCGGcuuGUGGGUUGUGGac -5' |
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| 11306 | 3' | -51.7 | NC_003083.1 | + | 58377 | 1.09 | 0.005545 |
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Target: 5'- gCACAAACGCCGAACACCCAACACCUGa -3' miRNA: 3'- -GUGUUUGCGGCUUGUGGGUUGUGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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