Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11307 | 5' | -48.9 | NC_003083.1 | + | 1669 | 0.69 | 0.967041 |
Target: 5'- uGCAGCugucc-UGCGUUaGGUGCGACg -3' miRNA: 3'- -CGUUGuuaacuGCGCAAaCCGCGCUG- -5' |
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11307 | 5' | -48.9 | NC_003083.1 | + | 115551 | 0.7 | 0.959861 |
Target: 5'- cGCAGCAAUucacUGACGC-UUUGaagauuauGCGCGAg -3' miRNA: 3'- -CGUUGUUA----ACUGCGcAAAC--------CGCGCUg -5' |
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11307 | 5' | -48.9 | NC_003083.1 | + | 34733 | 0.71 | 0.937402 |
Target: 5'- aGCAGCGcguugAUUGugGCGUgaacUGGUGCa-- -3' miRNA: 3'- -CGUUGU-----UAACugCGCAa---ACCGCGcug -5' |
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11307 | 5' | -48.9 | NC_003083.1 | + | 3619 | 0.72 | 0.894616 |
Target: 5'- --uGCAAUUG-CGCGUUUugaaacGGCGCGAg -3' miRNA: 3'- cguUGUUAACuGCGCAAA------CCGCGCUg -5' |
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11307 | 5' | -48.9 | NC_003083.1 | + | 64404 | 0.79 | 0.591313 |
Target: 5'- gGCGACGgcGUUGGCGaCGgugUUGGCGaCGGCg -3' miRNA: 3'- -CGUUGU--UAACUGC-GCa--AACCGC-GCUG- -5' |
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11307 | 5' | -48.9 | NC_003083.1 | + | 51446 | 0.83 | 0.371907 |
Target: 5'- cGCAACccGUUGugucGCGUGUUUGGCGUGACg -3' miRNA: 3'- -CGUUGu-UAAC----UGCGCAAACCGCGCUG- -5' |
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11307 | 5' | -48.9 | NC_003083.1 | + | 73720 | 1.14 | 0.005513 |
Target: 5'- uGCAACAAUUGACGCGUUUGGCGCGACg -3' miRNA: 3'- -CGUUGUUAACUGCGCAAACCGCGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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