Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11309 | 5' | -51.4 | NC_003083.1 | + | 30483 | 0.66 | 0.982026 |
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Target: 5'- aCAGGGUguggugauugcgucgUGCUCACCGugcgccgcuACAACCcgCa -3' miRNA: 3'- -GUCUCA---------------GCGAGUGGCu--------UGUUGGuaGg -5' |
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| 11309 | 5' | -51.4 | NC_003083.1 | + | 111986 | 0.66 | 0.976448 |
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Target: 5'- gCGGGGUCGCUUucaCGAcaAACUGUCUg -3' miRNA: 3'- -GUCUCAGCGAGug-GCUugUUGGUAGG- -5' |
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| 11309 | 5' | -51.4 | NC_003083.1 | + | 43245 | 0.67 | 0.967809 |
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Target: 5'- aCGGGGUCGUuguuccauUUGCCGuGCGcACCAUCg -3' miRNA: 3'- -GUCUCAGCG--------AGUGGCuUGU-UGGUAGg -5' |
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| 11309 | 5' | -51.4 | NC_003083.1 | + | 19998 | 0.67 | 0.967809 |
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Target: 5'- ---uGUUGCUCGCaaaCGAGCGGCUGUUCa -3' miRNA: 3'- gucuCAGCGAGUG---GCUUGUUGGUAGG- -5' |
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| 11309 | 5' | -51.4 | NC_003083.1 | + | 30680 | 0.67 | 0.96093 |
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Target: 5'- -cGuGUCGUauUUGCCGuGCGGCCAUUCg -3' miRNA: 3'- guCuCAGCG--AGUGGCuUGUUGGUAGG- -5' |
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| 11309 | 5' | -51.4 | NC_003083.1 | + | 93732 | 0.69 | 0.922955 |
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Target: 5'- --aAGUUGCUUuuuuGCUGAGCAauaaaugACCAUCCg -3' miRNA: 3'- gucUCAGCGAG----UGGCUUGU-------UGGUAGG- -5' |
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| 11309 | 5' | -51.4 | NC_003083.1 | + | 87856 | 0.71 | 0.861938 |
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Target: 5'- aGGGGUCGCUUGC---ACGACCAcgUCCg -3' miRNA: 3'- gUCUCAGCGAGUGgcuUGUUGGU--AGG- -5' |
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| 11309 | 5' | -51.4 | NC_003083.1 | + | 104177 | 0.74 | 0.680505 |
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Target: 5'- cUAGGGUCGCUCACUGugaAACAAUCAa-- -3' miRNA: 3'- -GUCUCAGCGAGUGGC---UUGUUGGUagg -5' |
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| 11309 | 5' | -51.4 | NC_003083.1 | + | 38043 | 0.83 | 0.25949 |
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Target: 5'- ---cGUCGCUCGCCGAGgAugCAUCCa -3' miRNA: 3'- gucuCAGCGAGUGGCUUgUugGUAGG- -5' |
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| 11309 | 5' | -51.4 | NC_003083.1 | + | 91958 | 1.13 | 0.003753 |
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Target: 5'- aCAGAGUCGCUCACCGAACAACCAUCCu -3' miRNA: 3'- -GUCUCAGCGAGUGGCUUGUUGGUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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