Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1131 | 3' | -54.2 | NC_000942.1 | + | 19881 | 0.66 | 0.582706 |
Target: 5'- aUCGCCAucuCCgGCACcuuGGGGCAgcacaGUGGa -3' miRNA: 3'- -GGUGGUu--GG-CGUGu--CCCCGUag---UAUC- -5' |
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1131 | 3' | -54.2 | NC_000942.1 | + | 12481 | 0.67 | 0.5373 |
Target: 5'- aCCACCuccUCGUAgGGGGGCGgagCAUu- -3' miRNA: 3'- -GGUGGuu-GGCGUgUCCCCGUa--GUAuc -5' |
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1131 | 3' | -54.2 | NC_000942.1 | + | 18731 | 0.67 | 0.515048 |
Target: 5'- uCCACCAcuuGCCGgGcCAGGGGU-UCAa-- -3' miRNA: 3'- -GGUGGU---UGGCgU-GUCCCCGuAGUauc -5' |
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1131 | 3' | -54.2 | NC_000942.1 | + | 30546 | 0.69 | 0.450793 |
Target: 5'- aCAgCGACCGCACAGcaGGGaAUUGUAGc -3' miRNA: 3'- gGUgGUUGGCGUGUC--CCCgUAGUAUC- -5' |
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1131 | 3' | -54.2 | NC_000942.1 | + | 24438 | 0.69 | 0.420352 |
Target: 5'- uCgGCCAGCUGCA-AGGGcGCuUCGUAGc -3' miRNA: 3'- -GgUGGUUGGCGUgUCCC-CGuAGUAUC- -5' |
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1131 | 3' | -54.2 | NC_000942.1 | + | 6418 | 0.71 | 0.336964 |
Target: 5'- gUCAUuuaCGGCCGCAUAGGGGCAa----- -3' miRNA: 3'- -GGUG---GUUGGCGUGUCCCCGUaguauc -5' |
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1131 | 3' | -54.2 | NC_000942.1 | + | 19472 | 0.83 | 0.047285 |
Target: 5'- gCCACCaAACCGUcCAGGGGCcUCAUAGc -3' miRNA: 3'- -GGUGG-UUGGCGuGUCCCCGuAGUAUC- -5' |
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1131 | 3' | -54.2 | NC_000942.1 | + | 19066 | 1.11 | 0.000433 |
Target: 5'- uCCACCAACCGCACAGGGGCAUCAUAGa -3' miRNA: 3'- -GGUGGUUGGCGUGUCCCCGUAGUAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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