Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11310 | 5' | -48.1 | NC_003083.1 | + | 92736 | 0.66 | 0.99911 |
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Target: 5'- aCCAG-GGC-AUGUGCGgcGCUuGUUGGg -3' miRNA: 3'- -GGUUaCCGaUGCACGUa-CGAuUAGCC- -5' |
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| 11310 | 5' | -48.1 | NC_003083.1 | + | 31447 | 0.66 | 0.998666 |
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Target: 5'- aCCAAUcGGCcACcggaccccaugGUGCuUGCUGAUgCGGa -3' miRNA: 3'- -GGUUA-CCGaUG-----------CACGuACGAUUA-GCC- -5' |
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| 11310 | 5' | -48.1 | NC_003083.1 | + | 3728 | 0.68 | 0.994591 |
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Target: 5'- cCCGAUGucc-CG-GCAUGUUGAUCGGu -3' miRNA: 3'- -GGUUACcgauGCaCGUACGAUUAGCC- -5' |
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| 11310 | 5' | -48.1 | NC_003083.1 | + | 71284 | 0.69 | 0.983685 |
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Target: 5'- aCCAGUGuGC-GCGUGCAUGUcaaAAUCa- -3' miRNA: 3'- -GGUUAC-CGaUGCACGUACGa--UUAGcc -5' |
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| 11310 | 5' | -48.1 | NC_003083.1 | + | 51629 | 0.7 | 0.981597 |
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Target: 5'- gCCGAUGGUgcCGUGgAcgcgGCUGGUCGc -3' miRNA: 3'- -GGUUACCGauGCACgUa---CGAUUAGCc -5' |
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| 11310 | 5' | -48.1 | NC_003083.1 | + | 12086 | 0.7 | 0.971209 |
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Target: 5'- aCAAUGGCguuCGUGuCGUGCgcGUCa- -3' miRNA: 3'- gGUUACCGau-GCAC-GUACGauUAGcc -5' |
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| 11310 | 5' | -48.1 | NC_003083.1 | + | 28957 | 0.71 | 0.968059 |
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Target: 5'- aCAAUGGCcgGCGUGCAaguauuUGCgucAUCGu -3' miRNA: 3'- gGUUACCGa-UGCACGU------ACGau-UAGCc -5' |
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| 11310 | 5' | -48.1 | NC_003083.1 | + | 14621 | 0.72 | 0.93903 |
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Target: 5'- uCCAAUGcGCUACauuGUGCGUGUU-AUCGu -3' miRNA: 3'- -GGUUAC-CGAUG---CACGUACGAuUAGCc -5' |
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| 11310 | 5' | -48.1 | NC_003083.1 | + | 43063 | 0.78 | 0.714775 |
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Target: 5'- gUCGGUGGCuUGCGUGCAUGCguAUUGa -3' miRNA: 3'- -GGUUACCG-AUGCACGUACGauUAGCc -5' |
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| 11310 | 5' | -48.1 | NC_003083.1 | + | 109698 | 1.13 | 0.007779 |
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Target: 5'- gCCAAUGGCUACGUGCAUGCUAAUCGGc -3' miRNA: 3'- -GGUUACCGAUGCACGUACGAUUAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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