Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11312 | 3' | -52.1 | NC_003083.1 | + | 56214 | 0.68 | 0.892321 |
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Target: 5'- gCGACGCCgguuaaacaaacacGCACGCGG-AAGcgGUGCa -3' miRNA: 3'- -GUUGCGG--------------CGUGCGUCgUUCuuUACGa -5' |
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| 11312 | 3' | -52.1 | NC_003083.1 | + | 9079 | 0.69 | 0.879927 |
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Target: 5'- aAGCGCCGCACGUuuggguguGGCAAauGAUGaCg -3' miRNA: 3'- gUUGCGGCGUGCG--------UCGUUcuUUAC-Ga -5' |
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| 11312 | 3' | -52.1 | NC_003083.1 | + | 115281 | 0.69 | 0.847998 |
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Target: 5'- uGGCGCCGUugGCGGCcacu--UGCg -3' miRNA: 3'- gUUGCGGCGugCGUCGuucuuuACGa -5' |
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| 11312 | 3' | -52.1 | NC_003083.1 | + | 83996 | 0.71 | 0.7937 |
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Target: 5'- aCAACGCCGUauACGUuuguGCAGcAAAUGCUu -3' miRNA: 3'- -GUUGCGGCG--UGCGu---CGUUcUUUACGA- -5' |
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| 11312 | 3' | -52.1 | NC_003083.1 | + | 66503 | 0.71 | 0.76421 |
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Target: 5'- cCAACGCCGCAcCGCugucugAGCGcGAAuUGCg -3' miRNA: 3'- -GUUGCGGCGU-GCG------UCGUuCUUuACGa -5' |
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| 11312 | 3' | -52.1 | NC_003083.1 | + | 113796 | 0.72 | 0.733534 |
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Target: 5'- aCAACG-CGCGCGCAGCAcGAGAUu-- -3' miRNA: 3'- -GUUGCgGCGUGCGUCGUuCUUUAcga -5' |
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| 11312 | 3' | -52.1 | NC_003083.1 | + | 115037 | 0.72 | 0.691302 |
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Target: 5'- aGGCGCagaCGCACGUcaAGCGAGAAAUGg- -3' miRNA: 3'- gUUGCG---GCGUGCG--UCGUUCUUUACga -5' |
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| 11312 | 3' | -52.1 | NC_003083.1 | + | 115231 | 1.09 | 0.004592 |
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Target: 5'- cCAACGCCGCACGCAGCAAGAAAUGCUg -3' miRNA: 3'- -GUUGCGGCGUGCGUCGUUCUUUACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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