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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11313 | 5' | -54.4 | NC_003084.1 | + | 100950 | 0.66 | 0.887663 |
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Target: 5'- cGCCGCAucGGUGUacgaaGGCUGcAGUCa -3' miRNA: 3'- aCGGCGUu-CCACAgg---UCGGCuUUAGc -5' |
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| 11313 | 5' | -54.4 | NC_003084.1 | + | 103418 | 0.66 | 0.880456 |
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Target: 5'- cGCC-CGAGG--UCCGGCacCGAAAUCGu -3' miRNA: 3'- aCGGcGUUCCacAGGUCG--GCUUUAGC- -5' |
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| 11313 | 5' | -54.4 | NC_003084.1 | + | 54921 | 0.7 | 0.727633 |
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Target: 5'- cGCCcggacgGUGAGGUGUgCAGCCGGuucUCGu -3' miRNA: 3'- aCGG------CGUUCCACAgGUCGGCUuu-AGC- -5' |
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| 11313 | 5' | -54.4 | NC_003084.1 | + | 41288 | 0.71 | 0.63328 |
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Target: 5'- aUGCUGCuAGGU-UCCAGCCGAGc--- -3' miRNA: 3'- -ACGGCGuUCCAcAGGUCGGCUUuagc -5' |
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| 11313 | 5' | -54.4 | NC_003084.1 | + | 60823 | 0.72 | 0.577221 |
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Target: 5'- cGCCGCGAGGUGguacggauguucacUCCGGCCa------ -3' miRNA: 3'- aCGGCGUUCCAC--------------AGGUCGGcuuuagc -5' |
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| 11313 | 5' | -54.4 | NC_003084.1 | + | 60841 | 0.73 | 0.518258 |
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Target: 5'- aUGUCGgaCAGcGG-GUCCAGCCGAAGUUGa -3' miRNA: 3'- -ACGGC--GUU-CCaCAGGUCGGCUUUAGC- -5' |
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| 11313 | 5' | -54.4 | NC_003084.1 | + | 7013 | 1.08 | 0.002948 |
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Target: 5'- gUGCCGCAAGGUGUCCAGCCGAAAUCGc -3' miRNA: 3'- -ACGGCGUUCCACAGGUCGGCUUUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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