Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11314 | 3' | -57.6 | NC_003084.1 | + | 66234 | 0.66 | 0.745293 |
Target: 5'- aCUGCGGCUuucgaugaggacgacGAACCGcaaGuCCAGAGCa- -3' miRNA: 3'- -GACGUCGA---------------CUUGGCc--CuGGUCUCGac -5' |
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11314 | 3' | -57.6 | NC_003084.1 | + | 53502 | 0.66 | 0.731314 |
Target: 5'- aCUGCgAGUUGGAgUGGGG-CGGAGCg- -3' miRNA: 3'- -GACG-UCGACUUgGCCCUgGUCUCGac -5' |
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11314 | 3' | -57.6 | NC_003084.1 | + | 51209 | 0.67 | 0.714105 |
Target: 5'- aUGUggugAGCUGAACCGGGGCUccauauagauuagAuuggguaaaugugguGAGCUGg -3' miRNA: 3'- gACG----UCGACUUGGCCCUGG-------------U---------------CUCGAC- -5' |
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11314 | 3' | -57.6 | NC_003084.1 | + | 69153 | 0.67 | 0.700801 |
Target: 5'- uUGgAGCUGAaaaaguugaaACUGGGAUCgGGGGUUGg -3' miRNA: 3'- gACgUCGACU----------UGGCCCUGG-UCUCGAC- -5' |
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11314 | 3' | -57.6 | NC_003084.1 | + | 28342 | 0.67 | 0.700801 |
Target: 5'- aUGCAGCc---CCGGGGCguGuGGCUGa -3' miRNA: 3'- gACGUCGacuuGGCCCUGguC-UCGAC- -5' |
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11314 | 3' | -57.6 | NC_003084.1 | + | 26802 | 0.67 | 0.690496 |
Target: 5'- -cGUAGCUGGACCacGGAaaCCAGcucAGCUGu -3' miRNA: 3'- gaCGUCGACUUGGc-CCU--GGUC---UCGAC- -5' |
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11314 | 3' | -57.6 | NC_003084.1 | + | 38766 | 0.68 | 0.638395 |
Target: 5'- gCUGCAcacccGCggGAACCGGGGCUgccgAGAGUg- -3' miRNA: 3'- -GACGU-----CGa-CUUGGCCCUGG----UCUCGac -5' |
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11314 | 3' | -57.6 | NC_003084.1 | + | 105320 | 0.69 | 0.53477 |
Target: 5'- -aGUA-CUGcGACCGGGugauACCGGAGCUGg -3' miRNA: 3'- gaCGUcGAC-UUGGCCC----UGGUCUCGAC- -5' |
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11314 | 3' | -57.6 | NC_003084.1 | + | 80120 | 0.7 | 0.514646 |
Target: 5'- aCUGCAGUucggccgauuUGAACCGGucGGCCAG-GUUGu -3' miRNA: 3'- -GACGUCG----------ACUUGGCC--CUGGUCuCGAC- -5' |
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11314 | 3' | -57.6 | NC_003084.1 | + | 85378 | 0.71 | 0.475382 |
Target: 5'- aUGCAGUUGGGCCGGGcGCgAGuaaAGCUc -3' miRNA: 3'- gACGUCGACUUGGCCC-UGgUC---UCGAc -5' |
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11314 | 3' | -57.6 | NC_003084.1 | + | 5727 | 0.71 | 0.437668 |
Target: 5'- -aGCAcCUGAugCGGGGCCcGGGCUu -3' miRNA: 3'- gaCGUcGACUugGCCCUGGuCUCGAc -5' |
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11314 | 3' | -57.6 | NC_003084.1 | + | 11242 | 0.72 | 0.419466 |
Target: 5'- -aGCAGCUGAAgCGGGAggaaaUGGAGCUc -3' miRNA: 3'- gaCGUCGACUUgGCCCUg----GUCUCGAc -5' |
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11314 | 3' | -57.6 | NC_003084.1 | + | 87220 | 0.72 | 0.393043 |
Target: 5'- --aCAGCUGAACCuGGACCuGGAGaCUGa -3' miRNA: 3'- gacGUCGACUUGGcCCUGG-UCUC-GAC- -5' |
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11314 | 3' | -57.6 | NC_003084.1 | + | 45854 | 0.74 | 0.32054 |
Target: 5'- uCUGCGGCuUGGGCUGGacCCGGGGCUGc -3' miRNA: 3'- -GACGUCG-ACUUGGCCcuGGUCUCGAC- -5' |
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11314 | 3' | -57.6 | NC_003084.1 | + | 7642 | 1.07 | 0.001634 |
Target: 5'- uCUGCAGCUGAACCGGGACCAGAGCUGc -3' miRNA: 3'- -GACGUCGACUUGGCCCUGGUCUCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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