miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
11316 5' -54.7 NC_003084.1 + 61280 0.67 0.810994
Target:  5'- gGGUGCaccGAGCggUGGCGUGGUGgucGUGUa -3'
miRNA:   3'- -UCGCGa--CUCGgaAUCGCACCGU---UACA- -5'
11316 5' -54.7 NC_003084.1 + 10049 0.68 0.752813
Target:  5'- cGgGUUGAGCCgaGGU-UGGCGGUGUg -3'
miRNA:   3'- uCgCGACUCGGaaUCGcACCGUUACA- -5'
11316 5' -54.7 NC_003084.1 + 79229 0.69 0.711533
Target:  5'- cGUGUUGAGCacguUUAGUGUGGCcgaaacGGUGUa -3'
miRNA:   3'- uCGCGACUCGg---AAUCGCACCG------UUACA- -5'
11316 5' -54.7 NC_003084.1 + 102069 0.69 0.711533
Target:  5'- cGGCGC-GAGCCgcGGUGaUGGCAAcGUu -3'
miRNA:   3'- -UCGCGaCUCGGaaUCGC-ACCGUUaCA- -5'
11316 5' -54.7 NC_003084.1 + 534 0.69 0.67975
Target:  5'- aAGCGCUGAGCgUUGGCcucaGCAcgGg -3'
miRNA:   3'- -UCGCGACUCGgAAUCGcac-CGUuaCa -5'
11316 5' -54.7 NC_003084.1 + 29516 0.71 0.593801
Target:  5'- cGGCGCUGuacGGUCUUGGCGguucagucGGCAAUa- -3'
miRNA:   3'- -UCGCGAC---UCGGAAUCGCa-------CCGUUAca -5'
11316 5' -54.7 NC_003084.1 + 14684 1.07 0.002899
Target:  5'- uAGCGCUGAGCCUUAGCGUGGCAAUGUu -3'
miRNA:   3'- -UCGCGACUCGGAAUCGCACCGUUACA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.