Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11317 | 3' | -54.2 | NC_003084.1 | + | 77591 | 0.66 | 0.922324 |
Target: 5'- --aUACUcuaauuggggACUGuuuuCCGUCGGUGCuACGAg -3' miRNA: 3'- guaAUGG----------UGGCu---GGCAGCCACG-UGCU- -5' |
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11317 | 3' | -54.2 | NC_003084.1 | + | 90836 | 0.66 | 0.916532 |
Target: 5'- --cUAUCGCCcACCGUUGGUGUguaaccgaacGCGGc -3' miRNA: 3'- guaAUGGUGGcUGGCAGCCACG----------UGCU- -5' |
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11317 | 3' | -54.2 | NC_003084.1 | + | 9635 | 0.66 | 0.916532 |
Target: 5'- aCAUUGCCACgGGacCCGUgGGUGaacCugGGg -3' miRNA: 3'- -GUAAUGGUGgCU--GGCAgCCAC---GugCU- -5' |
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11317 | 3' | -54.2 | NC_003084.1 | + | 472 | 0.66 | 0.91049 |
Target: 5'- -----aCACCGAaaccCCGUUGGcGCACGGc -3' miRNA: 3'- guaaugGUGGCU----GGCAGCCaCGUGCU- -5' |
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11317 | 3' | -54.2 | NC_003084.1 | + | 17538 | 0.67 | 0.890883 |
Target: 5'- -----gCACCGACgGUCGGUGguaAUGAg -3' miRNA: 3'- guaaugGUGGCUGgCAGCCACg--UGCU- -5' |
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11317 | 3' | -54.2 | NC_003084.1 | + | 25150 | 0.67 | 0.890883 |
Target: 5'- ---gACCAC--ACCGuUCGGUGCACa- -3' miRNA: 3'- guaaUGGUGgcUGGC-AGCCACGUGcu -5' |
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11317 | 3' | -54.2 | NC_003084.1 | + | 18077 | 0.67 | 0.87661 |
Target: 5'- ---aACCGgCGACCGUUGcGUaGUACGGu -3' miRNA: 3'- guaaUGGUgGCUGGCAGC-CA-CGUGCU- -5' |
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11317 | 3' | -54.2 | NC_003084.1 | + | 95489 | 0.67 | 0.87661 |
Target: 5'- aGUUACCACgauaCGGCCGUCcaguGGUGCucCGu -3' miRNA: 3'- gUAAUGGUG----GCUGGCAG----CCACGu-GCu -5' |
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11317 | 3' | -54.2 | NC_003084.1 | + | 16708 | 0.67 | 0.87661 |
Target: 5'- -uUUGCCACCcGCUGUCGGUugaGUuuaugGCGAu -3' miRNA: 3'- guAAUGGUGGcUGGCAGCCA---CG-----UGCU- -5' |
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11317 | 3' | -54.2 | NC_003084.1 | + | 64190 | 0.68 | 0.861418 |
Target: 5'- --gUGCCcacuuuCCGGCCGUCGGaucaGUGCGGc -3' miRNA: 3'- guaAUGGu-----GGCUGGCAGCCa---CGUGCU- -5' |
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11317 | 3' | -54.2 | NC_003084.1 | + | 23247 | 0.68 | 0.837014 |
Target: 5'- --gUACgACCgGGCCuucaagCGGUGCGCGAu -3' miRNA: 3'- guaAUGgUGG-CUGGca----GCCACGUGCU- -5' |
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11317 | 3' | -54.2 | NC_003084.1 | + | 91559 | 0.69 | 0.783148 |
Target: 5'- ---aACCACCGGUgGUCcgcuccugcgaGGUGCGCGAa -3' miRNA: 3'- guaaUGGUGGCUGgCAG-----------CCACGUGCU- -5' |
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11317 | 3' | -54.2 | NC_003084.1 | + | 81141 | 0.69 | 0.773616 |
Target: 5'- ---gGCCGCCGACCGUaGGU-CAuCGAu -3' miRNA: 3'- guaaUGGUGGCUGGCAgCCAcGU-GCU- -5' |
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11317 | 3' | -54.2 | NC_003084.1 | + | 61141 | 0.7 | 0.763952 |
Target: 5'- aCAcgACCACCacgccACCGcUCGGUGCACc- -3' miRNA: 3'- -GUaaUGGUGGc----UGGC-AGCCACGUGcu -5' |
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11317 | 3' | -54.2 | NC_003084.1 | + | 59073 | 0.71 | 0.703724 |
Target: 5'- --cUGCUGCUaGACCGUgaacaGGUGCGCGAa -3' miRNA: 3'- guaAUGGUGG-CUGGCAg----CCACGUGCU- -5' |
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11317 | 3' | -54.2 | NC_003084.1 | + | 99656 | 0.71 | 0.662167 |
Target: 5'- ---cGCCACCGGCCGUaagcCGGU-CACGu -3' miRNA: 3'- guaaUGGUGGCUGGCA----GCCAcGUGCu -5' |
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11317 | 3' | -54.2 | NC_003084.1 | + | 103898 | 0.72 | 0.620198 |
Target: 5'- gGUUACCACCGGCgGaccUGcGUGUACGAa -3' miRNA: 3'- gUAAUGGUGGCUGgCa--GC-CACGUGCU- -5' |
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11317 | 3' | -54.2 | NC_003084.1 | + | 10259 | 0.74 | 0.497076 |
Target: 5'- --gUACCGCaCGACuCGaaGGUGCACGAc -3' miRNA: 3'- guaAUGGUG-GCUG-GCagCCACGUGCU- -5' |
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11317 | 3' | -54.2 | NC_003084.1 | + | 67208 | 0.76 | 0.439613 |
Target: 5'- ---cACCACCGugaucuGCgCGUCGGUGUGCGAu -3' miRNA: 3'- guaaUGGUGGC------UG-GCAGCCACGUGCU- -5' |
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11317 | 3' | -54.2 | NC_003084.1 | + | 52725 | 0.78 | 0.32182 |
Target: 5'- aAUUGCCGCUGuacCCGaugaauUCGGUGCACGAg -3' miRNA: 3'- gUAAUGGUGGCu--GGC------AGCCACGUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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