Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11317 | 5' | -59.5 | NC_003084.1 | + | 11520 | 0.66 | 0.680508 |
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Target: 5'- -aUCGUugagCGCCGugAGGGUgCGUCAAUg -3' miRNA: 3'- ggAGCGa---GUGGCugUCCCG-GCGGUUG- -5' |
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| 11317 | 5' | -59.5 | NC_003084.1 | + | 25591 | 0.66 | 0.670393 |
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Target: 5'- gUCUCGCUaUACUGAUGGcguGGUCGgCCAACu -3' miRNA: 3'- -GGAGCGA-GUGGCUGUC---CCGGC-GGUUG- -5' |
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| 11317 | 5' | -59.5 | NC_003084.1 | + | 33343 | 0.67 | 0.650077 |
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Target: 5'- uCCUCGCcCAUC-ACAcGGGCCaccGUCGACa -3' miRNA: 3'- -GGAGCGaGUGGcUGU-CCCGG---CGGUUG- -5' |
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| 11317 | 5' | -59.5 | NC_003084.1 | + | 71190 | 0.67 | 0.639894 |
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Target: 5'- cCCUUGCuguuugugaaUCAgCGACAccuGcGGcCCGCCAACg -3' miRNA: 3'- -GGAGCG----------AGUgGCUGU---C-CC-GGCGGUUG- -5' |
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| 11317 | 5' | -59.5 | NC_003084.1 | + | 5606 | 0.69 | 0.490397 |
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Target: 5'- gCCUUgaGCUCAgcccUCGuCAGGGCCaCCAGCu -3' miRNA: 3'- -GGAG--CGAGU----GGCuGUCCCGGcGGUUG- -5' |
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| 11317 | 5' | -59.5 | NC_003084.1 | + | 6265 | 0.7 | 0.434986 |
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Target: 5'- ---aGCUCACCucgGGCAcGGGCCGCUggUa -3' miRNA: 3'- ggagCGAGUGG---CUGU-CCCGGCGGuuG- -5' |
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| 11317 | 5' | -59.5 | NC_003084.1 | + | 24569 | 0.7 | 0.434986 |
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Target: 5'- -aUCGCgUCACCaACGGGGuuGCCGc- -3' miRNA: 3'- ggAGCG-AGUGGcUGUCCCggCGGUug -5' |
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| 11317 | 5' | -59.5 | NC_003084.1 | + | 85385 | 0.77 | 0.180676 |
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Target: 5'- aCUCGCUguCCGAgaUGGGGCUGCCGAg -3' miRNA: 3'- gGAGCGAguGGCU--GUCCCGGCGGUUg -5' |
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| 11317 | 5' | -59.5 | NC_003084.1 | + | 17628 | 1.1 | 0.000761 |
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Target: 5'- cCCUCGCUCACCGACAGGGCCGCCAACu -3' miRNA: 3'- -GGAGCGAGUGGCUGUCCCGGCGGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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