Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11318 | 3' | -53.1 | NC_003084.1 | + | 19426 | 0.66 | 0.936693 |
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Target: 5'- cUGUCCGACuCGUAcaAGAGCUGCGguucucagaacgaauUCCAc -3' miRNA: 3'- -ACAGGUUG-GCGU--UCUUGGUGC---------------AGGUa -5' |
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| 11318 | 3' | -53.1 | NC_003084.1 | + | 33239 | 0.66 | 0.934625 |
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Target: 5'- gGUCCAGCUGCcaGAGAACCAa---CAg -3' miRNA: 3'- aCAGGUUGGCG--UUCUUGGUgcagGUa -5' |
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| 11318 | 3' | -53.1 | NC_003084.1 | + | 39795 | 0.66 | 0.934101 |
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Target: 5'- --aCCAACCGCAGcGGACCGaaacccuccagggUGUCCu- -3' miRNA: 3'- acaGGUUGGCGUU-CUUGGU-------------GCAGGua -5' |
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| 11318 | 3' | -53.1 | NC_003084.1 | + | 59882 | 0.67 | 0.898525 |
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Target: 5'- -aUCgAGCCGCAGGuACUGCGUCgGa -3' miRNA: 3'- acAGgUUGGCGUUCuUGGUGCAGgUa -5' |
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| 11318 | 3' | -53.1 | NC_003084.1 | + | 103662 | 0.67 | 0.884414 |
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Target: 5'- aGUCC-ACUGCA--GACC-CGUCCAc -3' miRNA: 3'- aCAGGuUGGCGUucUUGGuGCAGGUa -5' |
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| 11318 | 3' | -53.1 | NC_003084.1 | + | 81531 | 0.68 | 0.84406 |
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Target: 5'- aGUUgUAACCGUggGGcucgcgaACCACGUCCGg -3' miRNA: 3'- aCAG-GUUGGCGuuCU-------UGGUGCAGGUa -5' |
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| 11318 | 3' | -53.1 | NC_003084.1 | + | 64313 | 0.71 | 0.72014 |
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Target: 5'- -aUCCAGCgCGCAGuuGCuCACGUCCAc -3' miRNA: 3'- acAGGUUG-GCGUUcuUG-GUGCAGGUa -5' |
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| 11318 | 3' | -53.1 | NC_003084.1 | + | 15075 | 0.74 | 0.507769 |
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Target: 5'- cGUCCAGCUcaacCGAGAGCCGCGgaUCCAg -3' miRNA: 3'- aCAGGUUGGc---GUUCUUGGUGC--AGGUa -5' |
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| 11318 | 3' | -53.1 | NC_003084.1 | + | 21762 | 1.07 | 0.004583 |
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Target: 5'- aUGUCCAACCGCAAGAACCACGUCCAUg -3' miRNA: 3'- -ACAGGUUGGCGUUCUUGGUGCAGGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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