Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11318 | 5' | -57.3 | NC_003084.1 | + | 72510 | 0.66 | 0.764218 |
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Target: 5'- ---aGAGCCCGacgaaauGUUgGCCCGgGUCGAc -3' miRNA: 3'- cauaCUCGGGU-------CAGgCGGGUgCAGUU- -5' |
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| 11318 | 5' | -57.3 | NC_003084.1 | + | 30352 | 0.66 | 0.725311 |
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Target: 5'- -gGUGAGUUgCGGUCCGCCCGaGUUg- -3' miRNA: 3'- caUACUCGG-GUCAGGCGGGUgCAGuu -5' |
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| 11318 | 5' | -57.3 | NC_003084.1 | + | 58422 | 0.67 | 0.672569 |
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Target: 5'- --uUGAGCCCAGagUCGgugaauacgugcaCCCGCGUCAc -3' miRNA: 3'- cauACUCGGGUCa-GGC-------------GGGUGCAGUu -5' |
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| 11318 | 5' | -57.3 | NC_003084.1 | + | 62785 | 0.68 | 0.635767 |
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Target: 5'- -aAUGAagcccaagucGCCCAGUugauagaugugcaccUCGCCCACGUCu- -3' miRNA: 3'- caUACU----------CGGGUCA---------------GGCGGGUGCAGuu -5' |
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| 11318 | 5' | -57.3 | NC_003084.1 | + | 99486 | 0.68 | 0.631552 |
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Target: 5'- --uUGAGCCCAGcCCGCUCggacgAgGUCAu -3' miRNA: 3'- cauACUCGGGUCaGGCGGG-----UgCAGUu -5' |
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| 11318 | 5' | -57.3 | NC_003084.1 | + | 21719 | 1.06 | 0.001935 |
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Target: 5'- aGUAUGAGCCCAGUCCGCCCACGUCAAg -3' miRNA: 3'- -CAUACUCGGGUCAGGCGGGUGCAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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