Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1132 | 3' | -47.7 | NC_000942.1 | + | 20682 | 0.67 | 0.921056 |
Target: 5'- aUACCuugGGUAAUGUUACC---GGUGUGGc -3' miRNA: 3'- -GUGG---CCAUUGUAAUGGguuCCAUACC- -5' |
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1132 | 3' | -47.7 | NC_000942.1 | + | 30043 | 0.68 | 0.881709 |
Target: 5'- -cCCGGgGACAUgggacuuggcggugUGCCCGGGGacAUGGg -3' miRNA: 3'- guGGCCaUUGUA--------------AUGGGUUCCa-UACC- -5' |
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1132 | 3' | -47.7 | NC_000942.1 | + | 29962 | 0.68 | 0.875 |
Target: 5'- -cCCGGgGACAUgggacuggcggugUGCCCGGGGacAUGGg -3' miRNA: 3'- guGGCCaUUGUA-------------AUGGGUUCCa-UACC- -5' |
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1132 | 3' | -47.7 | NC_000942.1 | + | 9656 | 0.68 | 0.8583 |
Target: 5'- cCACCGGUGGCcgUcagcGCgCCAGGcGUAacuUGGc -3' miRNA: 3'- -GUGGCCAUUGuaA----UG-GGUUC-CAU---ACC- -5' |
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1132 | 3' | -47.7 | NC_000942.1 | + | 20628 | 1.13 | 0.001714 |
Target: 5'- aCACCGGUAACAUUACCCAAGGUAUGGg -3' miRNA: 3'- -GUGGCCAUUGUAAUGGGUUCCAUACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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