Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11320 | 3' | -53.7 | NC_003084.1 | + | 127 | 0.66 | 0.926144 |
Target: 5'- uGUUGUUCaGCGUGCACuCGUUCAGCGu -3' miRNA: 3'- -UAGUAAGgUGUGCGUGuGCAGGUCGC- -5' |
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11320 | 3' | -53.7 | NC_003084.1 | + | 59860 | 0.66 | 0.926144 |
Target: 5'- ----gUCCGCGCGCuGCAC-UCUAGCu -3' miRNA: 3'- uaguaAGGUGUGCG-UGUGcAGGUCGc -5' |
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11320 | 3' | -53.7 | NC_003084.1 | + | 15094 | 0.66 | 0.926144 |
Target: 5'- -gCGUggcgCCGCAU-CACAuCGUCCAGCu -3' miRNA: 3'- uaGUAa---GGUGUGcGUGU-GCAGGUCGc -5' |
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11320 | 3' | -53.7 | NC_003084.1 | + | 25862 | 0.66 | 0.916848 |
Target: 5'- -cCGggCCGCugGUgcugcauacaucgagGCugGUCCAGCu -3' miRNA: 3'- uaGUaaGGUGugCG---------------UGugCAGGUCGc -5' |
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11320 | 3' | -53.7 | NC_003084.1 | + | 59409 | 0.66 | 0.901657 |
Target: 5'- cUCAcuUUCCuugGCAuugcgagacuuuCGCGCACGUUUAGCGg -3' miRNA: 3'- uAGU--AAGG---UGU------------GCGUGUGCAGGUCGC- -5' |
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11320 | 3' | -53.7 | NC_003084.1 | + | 95092 | 0.67 | 0.857395 |
Target: 5'- ----aUCCACGCuCAU-CGUCCAGCGc -3' miRNA: 3'- uaguaAGGUGUGcGUGuGCAGGUCGC- -5' |
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11320 | 3' | -53.7 | NC_003084.1 | + | 93274 | 0.68 | 0.832174 |
Target: 5'- uAUCGUUCaGCACGauauCGUCCAGCu -3' miRNA: 3'- -UAGUAAGgUGUGCguguGCAGGUCGc -5' |
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11320 | 3' | -53.7 | NC_003084.1 | + | 97629 | 0.68 | 0.814352 |
Target: 5'- uUCAUcUCCAaaaACGUGCGCGgucUCCAGCu -3' miRNA: 3'- uAGUA-AGGUg--UGCGUGUGC---AGGUCGc -5' |
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11320 | 3' | -53.7 | NC_003084.1 | + | 977 | 0.69 | 0.805164 |
Target: 5'- -cCGUUUCACAuCGCGCACGUCgAaCGa -3' miRNA: 3'- uaGUAAGGUGU-GCGUGUGCAGgUcGC- -5' |
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11320 | 3' | -53.7 | NC_003084.1 | + | 34878 | 0.69 | 0.765809 |
Target: 5'- uGUCGUguggCCAgGCgucuucaGCACGCGaCCAGCGg -3' miRNA: 3'- -UAGUAa---GGUgUG-------CGUGUGCaGGUCGC- -5' |
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11320 | 3' | -53.7 | NC_003084.1 | + | 26015 | 0.7 | 0.756853 |
Target: 5'- cUCGggagCCcaaACGCGCACAaaaGUCCAGCc -3' miRNA: 3'- uAGUaa--GG---UGUGCGUGUg--CAGGUCGc -5' |
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11320 | 3' | -53.7 | NC_003084.1 | + | 80476 | 0.74 | 0.484954 |
Target: 5'- uGUCAacCCGCuucgugAUGCACAUGUCCAGCGc -3' miRNA: 3'- -UAGUaaGGUG------UGCGUGUGCAGGUCGC- -5' |
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11320 | 3' | -53.7 | NC_003084.1 | + | 51807 | 0.75 | 0.474073 |
Target: 5'- -cCGUUCCACGCGCGCACGUaauauuuuacuccCCuGUGc -3' miRNA: 3'- uaGUAAGGUGUGCGUGUGCA-------------GGuCGC- -5' |
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11320 | 3' | -53.7 | NC_003084.1 | + | 15789 | 0.75 | 0.465263 |
Target: 5'- -----cUCACGCGCAgCACGUCCGGCa -3' miRNA: 3'- uaguaaGGUGUGCGU-GUGCAGGUCGc -5' |
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11320 | 3' | -53.7 | NC_003084.1 | + | 12617 | 0.75 | 0.465263 |
Target: 5'- gGUCAggaCCGCcagaugGCGUACAUGUCCAGCa -3' miRNA: 3'- -UAGUaa-GGUG------UGCGUGUGCAGGUCGc -5' |
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11320 | 3' | -53.7 | NC_003084.1 | + | 25894 | 1.07 | 0.004184 |
Target: 5'- gAUCAUUCCACACGCACACGUCCAGCGu -3' miRNA: 3'- -UAGUAAGGUGUGCGUGUGCAGGUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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