Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11322 | 5' | -53 | NC_003084.1 | + | 29118 | 0.66 | 0.958085 |
Target: 5'- aGGAUGACgGAaaaacacgccuuuCAGAUCCGauuacggcGGUGCUg -3' miRNA: 3'- -CCUGCUGgCU-------------GUCUAGGUga------UCACGA- -5' |
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11322 | 5' | -53 | NC_003084.1 | + | 59588 | 0.66 | 0.941253 |
Target: 5'- gGGACGACUccucggugGACAcGAgcuaCAUUGGUGCa -3' miRNA: 3'- -CCUGCUGG--------CUGU-CUag--GUGAUCACGa -5' |
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11322 | 5' | -53 | NC_003084.1 | + | 85596 | 0.67 | 0.919985 |
Target: 5'- aGuCGGCCGAacugcccGGUCCGCUGGUGg- -3' miRNA: 3'- cCuGCUGGCUgu-----CUAGGUGAUCACga -5' |
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11322 | 5' | -53 | NC_003084.1 | + | 20008 | 0.67 | 0.914026 |
Target: 5'- -aACGugUaGAUGGGUCCAgUGGUGCg -3' miRNA: 3'- ccUGCugG-CUGUCUAGGUgAUCACGa -5' |
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11322 | 5' | -53 | NC_003084.1 | + | 98734 | 0.67 | 0.907812 |
Target: 5'- -uGCGACCGAUcccaGGGUgCACUGGaGCUc -3' miRNA: 3'- ccUGCUGGCUG----UCUAgGUGAUCaCGA- -5' |
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11322 | 5' | -53 | NC_003084.1 | + | 106452 | 0.68 | 0.887663 |
Target: 5'- uGGCGAguuugccucacCCGACGGAca-ACUGGUGCUa -3' miRNA: 3'- cCUGCU-----------GGCUGUCUaggUGAUCACGA- -5' |
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11322 | 5' | -53 | NC_003084.1 | + | 88700 | 0.68 | 0.880456 |
Target: 5'- aGAUGACCGAU-GA-CCaACUGGUGCc -3' miRNA: 3'- cCUGCUGGCUGuCUaGG-UGAUCACGa -5' |
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11322 | 5' | -53 | NC_003084.1 | + | 14557 | 0.68 | 0.873012 |
Target: 5'- cGGugGACCGACGcGggCCGaggucGUGCa -3' miRNA: 3'- -CCugCUGGCUGU-CuaGGUgau--CACGa -5' |
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11322 | 5' | -53 | NC_003084.1 | + | 94844 | 0.69 | 0.865336 |
Target: 5'- cGGCGGCCGGCcgcaaAGGUUCGCcaauGUGCg -3' miRNA: 3'- cCUGCUGGCUG-----UCUAGGUGau--CACGa -5' |
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11322 | 5' | -53 | NC_003084.1 | + | 14963 | 0.7 | 0.805689 |
Target: 5'- uGGACGAUUGauGCGGcgCCACgcuacAGUGCg -3' miRNA: 3'- -CCUGCUGGC--UGUCuaGGUGa----UCACGa -5' |
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11322 | 5' | -53 | NC_003084.1 | + | 68729 | 0.7 | 0.805689 |
Target: 5'- -cACGACCGACaccGGGUCCACUuucGUGg- -3' miRNA: 3'- ccUGCUGGCUG---UCUAGGUGAu--CACga -5' |
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11322 | 5' | -53 | NC_003084.1 | + | 29281 | 0.7 | 0.796421 |
Target: 5'- uGGACGcACgGACAGuuuGUUCACguugAGUGCa -3' miRNA: 3'- -CCUGC-UGgCUGUC---UAGGUGa---UCACGa -5' |
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11322 | 5' | -53 | NC_003084.1 | + | 28510 | 1.11 | 0.003034 |
Target: 5'- aGGACGACCGACAGAUCCACUAGUGCUa -3' miRNA: 3'- -CCUGCUGGCUGUCUAGGUGAUCACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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