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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11324 | 5' | -53.3 | NC_003084.1 | + | 38252 | 0.66 | 0.912816 |
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Target: 5'- --cGCCCAuAGaUGCGGGCCUGUuucaaucgCCAGc -3' miRNA: 3'- cacUGGGU-UCaACGUUCGGACA--------GGUC- -5' |
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| 11324 | 5' | -53.3 | NC_003084.1 | + | 95141 | 0.66 | 0.899781 |
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Target: 5'- cGUGAaaCCCAcgcccGGUcgUGCAAGCCg--CCGGg -3' miRNA: 3'- -CACU--GGGU-----UCA--ACGUUCGGacaGGUC- -5' |
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| 11324 | 5' | -53.3 | NC_003084.1 | + | 47430 | 0.67 | 0.885711 |
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Target: 5'- uUGGCCCGacGGUUGCAaauagAGacaCUGUUCGGc -3' miRNA: 3'- cACUGGGU--UCAACGU-----UCg--GACAGGUC- -5' |
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| 11324 | 5' | -53.3 | NC_003084.1 | + | 45544 | 0.67 | 0.870639 |
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Target: 5'- uUGAUgCAAGUUGUgcacgaccGGGCCaaGUCCAGg -3' miRNA: 3'- cACUGgGUUCAACG--------UUCGGa-CAGGUC- -5' |
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| 11324 | 5' | -53.3 | NC_003084.1 | + | 37739 | 1.09 | 0.003168 |
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Target: 5'- aGUGACCCAAGUUGCAAGCCUGUCCAGu -3' miRNA: 3'- -CACUGGGUUCAACGUUCGGACAGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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