Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11325 | 5' | -55.4 | NC_003084.1 | + | 99484 | 0.66 | 0.810994 |
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Target: 5'- -gAGCCCAGCCcgcucGGACGaGGUCaugCGCa- -3' miRNA: 3'- aaUCGGGUUGGu----UCUGC-CCAG---GUGaa -5' |
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| 11325 | 5' | -55.4 | NC_003084.1 | + | 7851 | 0.67 | 0.782576 |
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Target: 5'- gUGGUCCucACCAAa--GGGUCCACUc -3' miRNA: 3'- aAUCGGGu-UGGUUcugCCCAGGUGAa -5' |
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| 11325 | 5' | -55.4 | NC_003084.1 | + | 25395 | 0.68 | 0.67975 |
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Target: 5'- -gGGUCC-ACCGAGcACGGGUCC-Cg- -3' miRNA: 3'- aaUCGGGuUGGUUC-UGCCCAGGuGaa -5' |
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| 11325 | 5' | -55.4 | NC_003084.1 | + | 38025 | 0.69 | 0.669055 |
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Target: 5'- gUUAGCaCGACUuuaACGGGUCCACUUu -3' miRNA: 3'- -AAUCGgGUUGGuucUGCCCAGGUGAA- -5' |
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| 11325 | 5' | -55.4 | NC_003084.1 | + | 49420 | 0.71 | 0.53036 |
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Target: 5'- -gAGCaCAACCGAcauCGGGUCCACUUu -3' miRNA: 3'- aaUCGgGUUGGUUcu-GCCCAGGUGAA- -5' |
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| 11325 | 5' | -55.4 | NC_003084.1 | + | 68734 | 0.72 | 0.499548 |
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Target: 5'- gUUAGCaCGACCGAcacCGGGUCCACUUu -3' miRNA: 3'- -AAUCGgGUUGGUUcu-GCCCAGGUGAA- -5' |
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| 11325 | 5' | -55.4 | NC_003084.1 | + | 68589 | 0.79 | 0.178477 |
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Target: 5'- gUUAGCUCAACCGAcacCGGGUCCACUUu -3' miRNA: 3'- -AAUCGGGUUGGUUcu-GCCCAGGUGAA- -5' |
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| 11325 | 5' | -55.4 | NC_003084.1 | + | 46071 | 0.81 | 0.140204 |
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Target: 5'- aUGGUCCAACCGAGGCGGGcCCAaCUUc -3' miRNA: 3'- aAUCGGGUUGGUUCUGCCCaGGU-GAA- -5' |
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| 11325 | 5' | -55.4 | NC_003084.1 | + | 37961 | 1.02 | 0.004626 |
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Target: 5'- gUUAGCCCAACCAAGACGGGUCCACUUu -3' miRNA: 3'- -AAUCGGGUUGGUUCUGCCCAGGUGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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