Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11326 | 5' | -54.1 | NC_003084.1 | + | 63590 | 0.66 | 0.930657 |
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Target: 5'- cCGAuacuCUGGCCgGUUAACGGUgACg -3' miRNA: 3'- aGCUcauuGGCCGG-CAGUUGCCAgUG- -5' |
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| 11326 | 5' | -54.1 | NC_003084.1 | + | 90138 | 0.66 | 0.930126 |
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Target: 5'- -----aAACUGGCCGcgguaaaguucgaUCAGCGGUCAa -3' miRNA: 3'- agcucaUUGGCCGGC-------------AGUUGCCAGUg -5' |
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| 11326 | 5' | -54.1 | NC_003084.1 | + | 107694 | 0.67 | 0.894368 |
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Target: 5'- cCGAGcacGGgCGaGCCGUgGACGGUgGCa -3' miRNA: 3'- aGCUCa--UUgGC-CGGCAgUUGCCAgUG- -5' |
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| 11326 | 5' | -54.1 | NC_003084.1 | + | 88912 | 0.68 | 0.849422 |
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Target: 5'- gCGAGU-ACCGGCCcaCggUGGaCACg -3' miRNA: 3'- aGCUCAuUGGCCGGcaGuuGCCaGUG- -5' |
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| 11326 | 5' | -54.1 | NC_003084.1 | + | 99657 | 0.69 | 0.815217 |
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Target: 5'- aCGcc--ACCGGCCGUaagcCGGUCACg -3' miRNA: 3'- aGCucauUGGCCGGCAguu-GCCAGUG- -5' |
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| 11326 | 5' | -54.1 | NC_003084.1 | + | 77963 | 0.69 | 0.797025 |
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Target: 5'- uUUGGGUAACCGGCCcgaAAUGGUgGu -3' miRNA: 3'- -AGCUCAUUGGCCGGcagUUGCCAgUg -5' |
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| 11326 | 5' | -54.1 | NC_003084.1 | + | 17830 | 0.7 | 0.728906 |
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Target: 5'- aCGAGuUGGCCGuaCGUgGACGG-CACg -3' miRNA: 3'- aGCUC-AUUGGCcgGCAgUUGCCaGUG- -5' |
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| 11326 | 5' | -54.1 | NC_003084.1 | + | 54954 | 0.78 | 0.348022 |
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Target: 5'- uUCGuGUAuCCGGCCGgu-GCGGUCAUa -3' miRNA: 3'- -AGCuCAUuGGCCGGCaguUGCCAGUG- -5' |
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| 11326 | 5' | -54.1 | NC_003084.1 | + | 39760 | 1.09 | 0.003207 |
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Target: 5'- cUCGAGUAACCGGCCGUCAACGGUCACc -3' miRNA: 3'- -AGCUCAUUGGCCGGCAGUUGCCAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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